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Bibliographic Details
Main Author: Rubens Belfort-Neto
Format: Artículo científico
Language:en
Published: Academia Brasileira de Ciências 2007
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Online Access:https://www.redalyc.org/articulo.oa?id=32779113
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  • High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil Rubens Belfort-Neto Veronique Nussenblatt Luiz Rizzo Cristina Muccioli Claudio Silveira Robert Nussenblatt Assis Khan L. David Sibley Rubens Belfort Multidisciplinaria (Ciencias Naturales y Exactas) genotypes Toxoplasma gondii ocular toxoplasmosis Toxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of thecountry. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs andused molecular biological technique to determine the prevalence of infection and RFLP analysis to type the parasites.Seventeen out of 50 (34%) samples from the diaphragm and 33 out of 50 (66%) samples from the tongue demonstrateda positive PCR reaction for T. gondii and restriction analysis of four of the positive samples revealed that all had a type Igenotype at SAG2. However, when other unlinked loci were analyzed, these strains had a type III genotype at markersBTUB, SAG3, and GRA6. One of the strains (8T) had a type II allele at SAG3, indicating it has a combination ofalleles normally seen in the clonal lineages. Our sampling indicates a high prevalence of infection and suggests thatunusual genotypes of T. gondii are found in Brazil even among domesticated pigs. 2007 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32779113 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.79