Production of transgenic goat (Capra hircus) with human Granulocyte Colony Stimulating Factor (hG-CSF) gene in Brazil
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| Format: | Artículo científico |
| Langue: | en |
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Academia Brasileira de Ciências
2007
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| _version_ | 1876455609703858176 |
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| author | Vicente José de F. Freitas |
| author_facet | Vicente José de F. Freitas |
| contents | Production of transgenic goat (Capra hircus) with human Granulocyte Colony Stimulating Factor (hG-CSF) gene in Brazil Vicente José de F. Freitas Lyudmila E. Andreeva Guennadi A. Dvoryanchikov Edilson S. Lopes Dárcio I.A. Teixeira Luciene P.B. Dias Suely R.G. Avelar Raylene R. Moura Luciana M. Melo Alexsandra F. Pereira João B. Cajazeiras Maria L.L. Andrade Karlliely C. Almeida Francisco C. Sousa Irina A. Serova Antonio C.C. Carvalho Oleg L. Serov Multidisciplinaria (Ciencias Naturales y Exactas) hG CSF goat embryo transgenesis In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 adult undefined breed goats wereused as donors and recipients, respectively. Donors were estrus-synchronized with vaginal sponges and superovulatedby a treatment with 200 mg FSH given twice daily in decreasing doses over 3 days starting 48 h before sponge removal.Ovulation was induced by injecting 100 µg GnRH 36 h after sponge removal. The recipients also received an estrussynchronization treatment. Donors were mated with fertile Saanen bucks and, approximately 72 h after sponge removal,zygotes were recovered surgically by flushing oviducts. The recovered zygotes were briefly centrifuged to a reliablevisualization of the pronuclei. The DNA construct containing hG-CSF gene flanked by goat and bovine αs1-caseinsequences was injected into pronuclei of 129 zygotes. The microinjected embryos (3-6 per female) were transferred to27 recipients. Ten recipients became pregnant and 12 kids were born. One transgenic male founder was identified inthe group of kids. This is the first report of a birth of a transgenic goat in Latin America. 2007 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32779403 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.4 Vol.79 |
| format | Artículo científico |
| id | redalyc_32779403 |
| institution | Redalyc |
| language | en |
| publishDate | 2007 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Production of transgenic goat (Capra hircus) with human Granulocyte Colony Stimulating Factor (hG-CSF) gene in Brazil Vicente José de F. Freitas Multidisciplinaria (Ciencias Naturales y Exactas) hG CSF goat embryo transgenesis Production of transgenic goat (Capra hircus) with human Granulocyte Colony Stimulating Factor (hG-CSF) gene in Brazil Vicente José de F. Freitas Lyudmila E. Andreeva Guennadi A. Dvoryanchikov Edilson S. Lopes Dárcio I.A. Teixeira Luciene P.B. Dias Suely R.G. Avelar Raylene R. Moura Luciana M. Melo Alexsandra F. Pereira João B. Cajazeiras Maria L.L. Andrade Karlliely C. Almeida Francisco C. Sousa Irina A. Serova Antonio C.C. Carvalho Oleg L. Serov Multidisciplinaria (Ciencias Naturales y Exactas) hG CSF goat embryo transgenesis In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 adult undefined breed goats wereused as donors and recipients, respectively. Donors were estrus-synchronized with vaginal sponges and superovulatedby a treatment with 200 mg FSH given twice daily in decreasing doses over 3 days starting 48 h before sponge removal.Ovulation was induced by injecting 100 µg GnRH 36 h after sponge removal. The recipients also received an estrussynchronization treatment. Donors were mated with fertile Saanen bucks and, approximately 72 h after sponge removal,zygotes were recovered surgically by flushing oviducts. The recovered zygotes were briefly centrifuged to a reliablevisualization of the pronuclei. The DNA construct containing hG-CSF gene flanked by goat and bovine αs1-caseinsequences was injected into pronuclei of 129 zygotes. The microinjected embryos (3-6 per female) were transferred to27 recipients. Ten recipients became pregnant and 12 kids were born. One transgenic male founder was identified inthe group of kids. This is the first report of a birth of a transgenic goat in Latin America. 2007 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32779403 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.4 Vol.79 |
| title | Production of transgenic goat (Capra hircus) with human Granulocyte Colony Stimulating Factor (hG-CSF) gene in Brazil |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) hG CSF goat embryo transgenesis |
| url | https://www.redalyc.org/articulo.oa?id=32779403 |