Inhibition of caspase-8 activity reduces IFN-y expression by T cells from Leishmania major infection
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| Format: | Artículo científico |
| Langue: | en |
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Academia Brasileira de Ciências
2008
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| _version_ | 1876459677245505536 |
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| author | Wânia F. Pereira |
| author_facet | Wânia F. Pereira |
| contents | Inhibition of caspase-8 activity reduces IFN-y expression by T cells from Leishmania major infection Wânia F. Pereira Landi V.C. Guillermo Flávia L. Ribeiro-Gomes Marcela de Freitas Lopes Multidisciplinaria (Ciencias Naturales y Exactas) IFN γ caspase apoptosis CD8 T cells Following infection with Leishmania major, T cell activation and apoptosis can be detected in draining lymph nodesof C57BL/6-infected mice. We investigated the mechanisms involved in apoptosis and cytokine expression followingT cell activation. After twoweeks of infection, apoptotic T cells were not detected in draining lymph nodes but activationwith anti-CD3 induced apoptosis in both CD4 and CD8 T cells. Treatment with anti-Fas Ligand, caspase-8 or caspase-9 inhibitors did not block activation-induced T-cell death. We also investigated whether the blockade of caspase-8activity would affect the expression of type-1 or type-2 cytokines. At early stages of infection, both CD4 and CD8T cells expressed IFN-γ upon activation. Treatment with the caspase-8 inhibitor zIETD-fmk (benzyl-oxycarbonyl-Ile-Glu(OMe)-Thr-Asp(OMe)-fluoromethyl ketone) reduced the proportion of CD8 T cells and IFN-γ expression in bothCD4 and CD8 T cells. We conclude that a non apoptotic role of caspase-8 activity may be required for T cell-mediatedtype-1 responses during L. major infection. 2008 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32780108 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.80 |
| format | Artículo científico |
| id | redalyc_32780108 |
| institution | Redalyc |
| language | en |
| publishDate | 2008 |
| publisher | Academia Brasileira de Ciências |
| spellingShingle | Inhibition of caspase-8 activity reduces IFN-y expression by T cells from Leishmania major infection Wânia F. Pereira Multidisciplinaria (Ciencias Naturales y Exactas) IFN γ caspase apoptosis CD8 T cells Inhibition of caspase-8 activity reduces IFN-y expression by T cells from Leishmania major infection Wânia F. Pereira Landi V.C. Guillermo Flávia L. Ribeiro-Gomes Marcela de Freitas Lopes Multidisciplinaria (Ciencias Naturales y Exactas) IFN γ caspase apoptosis CD8 T cells Following infection with Leishmania major, T cell activation and apoptosis can be detected in draining lymph nodesof C57BL/6-infected mice. We investigated the mechanisms involved in apoptosis and cytokine expression followingT cell activation. After twoweeks of infection, apoptotic T cells were not detected in draining lymph nodes but activationwith anti-CD3 induced apoptosis in both CD4 and CD8 T cells. Treatment with anti-Fas Ligand, caspase-8 or caspase-9 inhibitors did not block activation-induced T-cell death. We also investigated whether the blockade of caspase-8activity would affect the expression of type-1 or type-2 cytokines. At early stages of infection, both CD4 and CD8T cells expressed IFN-γ upon activation. Treatment with the caspase-8 inhibitor zIETD-fmk (benzyl-oxycarbonyl-Ile-Glu(OMe)-Thr-Asp(OMe)-fluoromethyl ketone) reduced the proportion of CD8 T cells and IFN-γ expression in bothCD4 and CD8 T cells. We conclude that a non apoptotic role of caspase-8 activity may be required for T cell-mediatedtype-1 responses during L. major infection. 2008 artículo científico 0001-3765 https://www.redalyc.org/articulo.oa?id=32780108 en http://www.redalyc.org/revista.oa?id=327 Anais da Academia Brasileira de Ciências application/pdf Academia Brasileira de Ciências Anais da Academia Brasileira de Ciências (Brasil) Num.1 Vol.80 |
| title | Inhibition of caspase-8 activity reduces IFN-y expression by T cells from Leishmania major infection |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) IFN γ caspase apoptosis CD8 T cells |
| url | https://www.redalyc.org/articulo.oa?id=32780108 |