Effect of naringenin, hesperetin and their glycosides forms on the replication of the 17D strain of yellow fever virus
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| Format: | Artículo científico |
| Language: | en |
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Universidad de los Andes
2015
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| author | Mariana Castrillo |
| author_facet | Mariana Castrillo |
| contents | Effect of naringenin, hesperetin and their glycosides forms on the replication of the 17D strain of yellow fever virus Mariana Castrillo Tania Córdova Gustavo Cabrera Morella Rodríguez-Ortega Medicina Antiviral Flavanones Flavivirus Yellow Fever Virus 17D Infections produced by flaviviruses are threats to public health worldwide. The absence of effective drugs treatment makes imperative the quest for efficient antivirals. Using a plaque inhibition assay we studied the antiviral action of the noncytotoxic flavanones naringenin, hesperetin, and their glycoside forms on the replication of the 17D strain of yellow fever virus (YFV17D). Using plaque assay the glycosylated forms did not show antiviral effect at the highest concentration used, naringenin and hesperetin reduced the plaque size and the infectious titer up to 85.51% and 100% respectively at the maximum concentration employed. The effective dose (ED50) of naringenin (0.0013 µM), calculated by plaque assay, was approximately tenfold lower than hesperetin (ED50 0.01 µM), and neither showed any virucidal effect. Evaluation of kinetic entrance of YFV to cell showed that after 2h of infection 80% of the virus was already into the cell, but in the presence of naringenin or hesperetin, this entrance were inhibited to 48.58% and 55.2% respectively (p≤ 0.05). Study of molecular parameters of naringenin and hesperetin demonstrated that naringenin Log P = -1.56 is ten times higher than hesperetin (Log P = -2.56), and therefore less hydrophilic and probably more effective in passing through membranes, which could be related to its ability to interfere with the viral replication beside the entrance level. 2015 artículo científico 2477-9369 https://www.redalyc.org/articulo.oa?id=331341624005 en http://www.redalyc.org/revista.oa?id=3313 Avances en Biomedicina application/pdf Universidad de los Andes Avances en Biomedicina (República Bolivariana de Venezuela) Num.2 Vol.4 |
| format | Artículo científico |
| id | redalyc_331341624005 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Universidad de los Andes |
| spellingShingle | Effect of naringenin, hesperetin and their glycosides forms on the replication of the 17D strain of yellow fever virus Mariana Castrillo Medicina Antiviral Flavanones Flavivirus Yellow Fever Virus 17D Effect of naringenin, hesperetin and their glycosides forms on the replication of the 17D strain of yellow fever virus Mariana Castrillo Tania Córdova Gustavo Cabrera Morella Rodríguez-Ortega Medicina Antiviral Flavanones Flavivirus Yellow Fever Virus 17D Infections produced by flaviviruses are threats to public health worldwide. The absence of effective drugs treatment makes imperative the quest for efficient antivirals. Using a plaque inhibition assay we studied the antiviral action of the noncytotoxic flavanones naringenin, hesperetin, and their glycoside forms on the replication of the 17D strain of yellow fever virus (YFV17D). Using plaque assay the glycosylated forms did not show antiviral effect at the highest concentration used, naringenin and hesperetin reduced the plaque size and the infectious titer up to 85.51% and 100% respectively at the maximum concentration employed. The effective dose (ED50) of naringenin (0.0013 µM), calculated by plaque assay, was approximately tenfold lower than hesperetin (ED50 0.01 µM), and neither showed any virucidal effect. Evaluation of kinetic entrance of YFV to cell showed that after 2h of infection 80% of the virus was already into the cell, but in the presence of naringenin or hesperetin, this entrance were inhibited to 48.58% and 55.2% respectively (p≤ 0.05). Study of molecular parameters of naringenin and hesperetin demonstrated that naringenin Log P = -1.56 is ten times higher than hesperetin (Log P = -2.56), and therefore less hydrophilic and probably more effective in passing through membranes, which could be related to its ability to interfere with the viral replication beside the entrance level. 2015 artículo científico 2477-9369 https://www.redalyc.org/articulo.oa?id=331341624005 en http://www.redalyc.org/revista.oa?id=3313 Avances en Biomedicina application/pdf Universidad de los Andes Avances en Biomedicina (República Bolivariana de Venezuela) Num.2 Vol.4 |
| title | Effect of naringenin, hesperetin and their glycosides forms on the replication of the 17D strain of yellow fever virus |
| topic | Medicina Antiviral Flavanones Flavivirus Yellow Fever Virus 17D |
| url | https://www.redalyc.org/articulo.oa?id=331341624005 |