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| Format: | Artículo científico |
| Language: | en |
| Published: |
Instituto Nacional de Salud
2006
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| Online Access: | https://www.redalyc.org/articulo.oa?id=36323406 |
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Table of Contents:
- Efecto citotóxico selectivo in vitro de muricin H(acetogenina de Annona muricata) en cultivos celulares de cáncer de pulmón Angel Quispe M. David Zavala C. José Rojas C. Margarita Posso R. Abraham Vaisberg W. Salud Phytotherapy Cytotoxicity Cell culture Medicinal plants Antineoplastic agents Objectives: To determine the selective cytotoxic activity of muricin H on a H460 cell line (non-small cell lung cancer).Materials and methods: H460 and 3T3 cell lines (normal murine fibroblasts) were exposed to six concentrations ofmuricin H (62,5; 15,6; 3,9; 0,98; 0,24; 0,06 µg/mL) and to equal concentrations of 5-fluorouracil (5-FU) used as a positivecontrol. Growth percentages at 48 hours were measured, and then the half-maximal inhibitory concentration (IC50)was determined using linear regression analysis, and Pearson correlation coefficients were calculated. Finally, the selectivityindex for each sample was calculated. Results: IC50 values for muricin H, expressed in µg/mL were as follows:<0,06 (r= -0,96; p<0,05) for the H5460 cell line, and 6,16 (r= -0,96; p<0,025) for the 3T3 cell line. IC50 with 5-FU were0,46 (r= 0,95; p<0,005) for H460, and 0,29 (r= -0,88 P= 0,01) for 3T3. Selectivity indexes for muricin H and 5-FU were<102,6 and 0,63, respectively. Conclusions: In vitro selective cytotoxic activity of muricin H was proven, since it had agreater cytotoxic effect for H460 cell line, and lesser effect upon the 3T3 cell line when compared to 5-FU. 2006 artículo científico 1726-4642 https://www.redalyc.org/articulo.oa?id=36323406 en http://www.redalyc.org/revista.oa?id=363 Revista Peruana de Medicina Experimental y Salud Pública application/pdf Instituto Nacional de Salud Revista Peruana de Medicina Experimental y Salud Pública (Perú) Num.4 Vol.23