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| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad Nacional Autónoma de México
2002
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| Online Access: | https://www.redalyc.org/articulo.oa?id=37018401 |
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Table of Contents:
- Determination of cadmium and zinc and its relationship to metallothionein levels in swine kidney Carmen Frasquillo Félix Refugio Robles Burgueño Martín Jara Marini Leticia García Rico Ciencias de la Tierra zinc cadmium metallothionein environment indicator Cadmium is considered a hazardous environment pollutant. Animals exposed to this metal showcadmium accumulation mainly in their kidneys. Over 60 % of cadmium binds to metallothioneins,i.e., proteins that act as detoxification agents. The purpose of this study was to isolatemetallothioneins in swine kidneys and to determine cadmium, zinc, and copper link with thisprotein. Twenty kidneys were selected and total concentration of cadmium, zinc, and copperwere analyzed by atomic absorption spectroscopy and total metallothioneins using the silversaturation method. Metallothioneins were isolated by gel filtration and ion exchange chromatography.AAS-graphite furnace was used to monitor cadmium, zinc, and copper in eluted fractions.Total concentrations of cadmium, zinc, copper, and metallothioneins were 1.20-2.40 µg/g,20.37-39.82 µg/g, 3.99-14.80 µg/g, and 4.26-101.68 µg/g, respectively. Results show a positivecorrelation between metallothioneins levels and each of the metals; a Zn-thionein and a Cdthioneinwere also identified. 2002 artículo científico 0188-4999 https://www.redalyc.org/articulo.oa?id=37018401 en http://www.redalyc.org/revista.oa?id=370 Revista Internacional de Contaminación Ambiental application/pdf Universidad Nacional Autónoma de México Revista Internacional de Contaminación Ambiental (México) Num.4 Vol.18