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| Format: | Artículo científico |
| Langue: | en |
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Universidad Nacional Autónoma de México
1988
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| Accès en ligne: | https://www.redalyc.org/articulo.oa?id=37040105 |
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- A three way differential staining protocol for the analysis of SCEs during three successive cell divisions in murine bone marrow cells in vivo Pedro Morales Ramírez Teresita Vallarino Kelly Regina Rodríguez Reyes Ciencias de la Tierra A method for the analysis of sister diromatid exchanges (SCEs) occurring in thrce subsequentcell divisions in uivo, was developed in murine bone marrow cells. The method was basedon the three-way differential staining of sister chromatids. Mice were injected twice ¡.p.with BrdU adsorbed to activated charcoal; the fint dose at O h (fint cell cycle) was variedbetween 0.1 and 0.25 mg of BrdU/g of body weight and the second dose at 8 h (secondand third cell cycles), was either 1.0, 1.33 or 1.5 mg of BrdU/g of body weight.Animals were injected with colchicine (¡.p.) two houn before sacrificing and chromosomeprepations were obtained by the usual method. After sister chromatid differentiation bythe method of fluomscence plus Giemsa the cells were scored for well and poor differentialstaining and in some cases for SCE frequency in three subsequent cell divisions.The results indicate that a higher percentage of well-differentiated three way stained cellswere obtained with the combination of BrdU doses of 0.2 and 1.5 mg/g of body weight.The very low basal frequency of SCE observed in the first cell cycle (0.31-0.46) probablyincreases the sensitivity to detected the mutagenic effect by SCE method. This methodcan be used for both the analysis of in uiuo penistence of lesions involved in SCE in,ductionas well as the detection of sinergism between the mutagen action and the BrdU incorporatioato DNA. 1988 artículo científico 0188-4999 https://www.redalyc.org/articulo.oa?id=37040105 en http://www.redalyc.org/revista.oa?id=370 Revista Internacional de Contaminación Ambiental application/pdf Universidad Nacional Autónoma de México Revista Internacional de Contaminación Ambiental (México) Num.1 Vol.4