GENOTOXIC EFFECT OF AZINPHOS METHYL IN BACTERIA AND IN HUMAN LYMPHOCYTE CULTURES AFTER PLANT ACTIVATION
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Universidad Nacional Autónoma de México
2015
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| _version_ | 1876487080260927488 |
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| author | Sandra GÓMEZ-ARROYO |
| author_facet | Sandra GÓMEZ-ARROYO |
| contents | GENOTOXIC EFFECT OF AZINPHOS METHYL IN BACTERIA AND IN HUMAN LYMPHOCYTE CULTURES AFTER PLANT ACTIVATION Sandra GÓMEZ-ARROYO Liliana SÁNCHEZ-ESTRADA Selene ANDRADE-MORALES Josefina CORTÉS-ESLAVA Rafael VILLALOBOS-PIETRINI Ciencias de la Tierra Azinphos methyl plant metabolism Salmonella typhimurium human lymphocyte cultures sister chromatid exchange The evaluation of the potential risk of pesticides applied to crops consumed by humans in Mexico is appropriate and necessary because plant pro-mutagenic transformation in toxic metabolites and their subsequent incorporation involve a risk for health when such crops are ingested. Plant metabolism of agricultural insecticides produces compounds that could be introduced in the food chain, increasing the contamination and poisoning risk by agrochemical metabolism. In this study we evaluated the effect of the organophosphorus insecticide azinphos methyl transformed by S10 fraction of broad bean (Vicia faba), using as indicator of mutagenic damage the reverse mutation of Salmonella typhimurium strains TA98 and TA100 and the sister chromatid exchange (SCE) in human lymphocyte cultures. Results of mutagenicity showed that when Salmonella TA98 and TA100 strains were treated directly with azinphos methyl, negative response was obtained. The same occurred with human lymphocytes tested directly with this insecticide. When Vicia faba S10 enzymatic mix was added, there was a mutagenic response in both Salmonella strains. These results suggest that the mechanisms to induce mutations by azinphos methyl were frameshift mutation (TA98 strain) as well as pair bases substitution (TA100 strain). Likewise, SCE production was significant and dose-response relationship was observed in human lymphocyte cultures. The cell kinetics (M1, M2 and M3 cells), the replication index and the mitotic index are also analyzed. Only in the treatments with S10 fraction the effects were observed. At the highest concentration mitotic inhibition was produced. 2015 artículo científico 0188-4999 https://www.redalyc.org/articulo.oa?id=37041042002 en http://www.redalyc.org/revista.oa?id=370 Revista Internacional de Contaminación Ambiental application/pdf Universidad Nacional Autónoma de México Revista Internacional de Contaminación Ambiental (México) Num.3 Vol.31 |
| format | Artículo científico |
| id | redalyc_37041042002 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Universidad Nacional Autónoma de México |
| spellingShingle | GENOTOXIC EFFECT OF AZINPHOS METHYL IN BACTERIA AND IN HUMAN LYMPHOCYTE CULTURES AFTER PLANT ACTIVATION Sandra GÓMEZ-ARROYO Ciencias de la Tierra Azinphos methyl plant metabolism Salmonella typhimurium human lymphocyte cultures sister chromatid exchange GENOTOXIC EFFECT OF AZINPHOS METHYL IN BACTERIA AND IN HUMAN LYMPHOCYTE CULTURES AFTER PLANT ACTIVATION Sandra GÓMEZ-ARROYO Liliana SÁNCHEZ-ESTRADA Selene ANDRADE-MORALES Josefina CORTÉS-ESLAVA Rafael VILLALOBOS-PIETRINI Ciencias de la Tierra Azinphos methyl plant metabolism Salmonella typhimurium human lymphocyte cultures sister chromatid exchange The evaluation of the potential risk of pesticides applied to crops consumed by humans in Mexico is appropriate and necessary because plant pro-mutagenic transformation in toxic metabolites and their subsequent incorporation involve a risk for health when such crops are ingested. Plant metabolism of agricultural insecticides produces compounds that could be introduced in the food chain, increasing the contamination and poisoning risk by agrochemical metabolism. In this study we evaluated the effect of the organophosphorus insecticide azinphos methyl transformed by S10 fraction of broad bean (Vicia faba), using as indicator of mutagenic damage the reverse mutation of Salmonella typhimurium strains TA98 and TA100 and the sister chromatid exchange (SCE) in human lymphocyte cultures. Results of mutagenicity showed that when Salmonella TA98 and TA100 strains were treated directly with azinphos methyl, negative response was obtained. The same occurred with human lymphocytes tested directly with this insecticide. When Vicia faba S10 enzymatic mix was added, there was a mutagenic response in both Salmonella strains. These results suggest that the mechanisms to induce mutations by azinphos methyl were frameshift mutation (TA98 strain) as well as pair bases substitution (TA100 strain). Likewise, SCE production was significant and dose-response relationship was observed in human lymphocyte cultures. The cell kinetics (M1, M2 and M3 cells), the replication index and the mitotic index are also analyzed. Only in the treatments with S10 fraction the effects were observed. At the highest concentration mitotic inhibition was produced. 2015 artículo científico 0188-4999 https://www.redalyc.org/articulo.oa?id=37041042002 en http://www.redalyc.org/revista.oa?id=370 Revista Internacional de Contaminación Ambiental application/pdf Universidad Nacional Autónoma de México Revista Internacional de Contaminación Ambiental (México) Num.3 Vol.31 |
| title | GENOTOXIC EFFECT OF AZINPHOS METHYL IN BACTERIA AND IN HUMAN LYMPHOCYTE CULTURES AFTER PLANT ACTIVATION |
| topic | Ciencias de la Tierra Azinphos methyl plant metabolism Salmonella typhimurium human lymphocyte cultures sister chromatid exchange |
| url | https://www.redalyc.org/articulo.oa?id=37041042002 |