ACUTE TOXICITY AND SUBLETHAL EFFECTS ON MACROMOLECULES CONCENTRATION, CALORIC CONTENT, AND LIPID PEROXIDATION DURING EXOGENOUS-FEEDING OF Danio rerio LARVAE EXPOSED TO Cu2+
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidad Nacional Autónoma de México
2015
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| _version_ | 1876485841527767040 |
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| author | Jesús RODRÍGUEZ-ESTRADA |
| author_facet | Jesús RODRÍGUEZ-ESTRADA |
| contents | ACUTE TOXICITY AND SUBLETHAL EFFECTS ON MACROMOLECULES CONCENTRATION, CALORIC CONTENT, AND LIPID PEROXIDATION DURING EXOGENOUS-FEEDING OF Danio rerio LARVAE EXPOSED TO Cu2+ Jesús RODRÍGUEZ-ESTRADA Alma Socorro SOBRINO-FIGUEROA Fernando MARTÍNEZ-JERÓNIMO Ciencias de la Tierra Zebrafish biomarkers toxic metals oxidative stress early life stages Copper is an essential micronutrient, involved as coenzyme in different metabolic processes. Due to its toxic effect, copper is also used as algicide and herbicide. Residual concentrations of this metal are released into aquatic environments as effluents from mining activities, industrial applications, and other sources, producing toxic effects on the aquatic biota. Although Danio rerio (zebrafish) embryos and larvae have been broadly used as surrogate species to assess toxic effects in water, scarce information exists on copper’s effects on fish larvae starting to feed exogenously (after yolk sac depletion). For this reason, the toxic effect of Cu on exogenous feeding larvae was assessed. Acute toxicity (96 h) was determined in 10 and 20 day post-fertilization (dpf) larvae. Additionally, the effect of sublethal Cu concentrations on the main macromolecules concentration, caloric content, and lipid peroxidation was also determined in 10 dpf larvae, as these were the most sensitive during acute exposure. The median lethal concentration (LC50) and confidence limit values (P = 95 %) were 148.52 ± 13.5 and 274.20 ± 40.21 μg/L for 10 and 20 dpf larvae, respectively. The caloric content, glycogen, and lipid concentration were markedly reduced in the 10 dpf larvae exposed to Cu. The malondialdehyde concentration was significantly modified in D. rerio larvae exposed during 48 h to the toxicant. Cu2+ concentrations as low as 16.04 and32.08 μg/L produced biochemical impairment in D. rerio exogenous feeding larvae. Our results warn about harmful effects on the aquatic biota exposed to Cu2+, because its concentrations in polluted waters can be higher than those assayed here. 2015 artículo científico 0188-4999 https://www.redalyc.org/articulo.oa?id=37043336007 https://www.redalyc.org/journal/370/37043336007/ https://www.redalyc.org/journal/370/37043336007/html/ https://www.redalyc.org/journal/370/37043336007/37043336007.epub https://www.redalyc.org/journal/370/37043336007/movil en http://www.redalyc.org/revista.oa?id=370 Revista Internacional de Contaminación Ambiental application/pdf Universidad Nacional Autónoma de México Revista Internacional de Contaminación Ambiental (México) Num.4 Vol.31 |
| format | Artículo científico |
| id | redalyc_37043336007 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Universidad Nacional Autónoma de México |
| spellingShingle | ACUTE TOXICITY AND SUBLETHAL EFFECTS ON MACROMOLECULES CONCENTRATION, CALORIC CONTENT, AND LIPID PEROXIDATION DURING EXOGENOUS-FEEDING OF Danio rerio LARVAE EXPOSED TO Cu2+ Jesús RODRÍGUEZ-ESTRADA Ciencias de la Tierra Zebrafish biomarkers toxic metals oxidative stress early life stages ACUTE TOXICITY AND SUBLETHAL EFFECTS ON MACROMOLECULES CONCENTRATION, CALORIC CONTENT, AND LIPID PEROXIDATION DURING EXOGENOUS-FEEDING OF Danio rerio LARVAE EXPOSED TO Cu2+ Jesús RODRÍGUEZ-ESTRADA Alma Socorro SOBRINO-FIGUEROA Fernando MARTÍNEZ-JERÓNIMO Ciencias de la Tierra Zebrafish biomarkers toxic metals oxidative stress early life stages Copper is an essential micronutrient, involved as coenzyme in different metabolic processes. Due to its toxic effect, copper is also used as algicide and herbicide. Residual concentrations of this metal are released into aquatic environments as effluents from mining activities, industrial applications, and other sources, producing toxic effects on the aquatic biota. Although Danio rerio (zebrafish) embryos and larvae have been broadly used as surrogate species to assess toxic effects in water, scarce information exists on copper’s effects on fish larvae starting to feed exogenously (after yolk sac depletion). For this reason, the toxic effect of Cu on exogenous feeding larvae was assessed. Acute toxicity (96 h) was determined in 10 and 20 day post-fertilization (dpf) larvae. Additionally, the effect of sublethal Cu concentrations on the main macromolecules concentration, caloric content, and lipid peroxidation was also determined in 10 dpf larvae, as these were the most sensitive during acute exposure. The median lethal concentration (LC50) and confidence limit values (P = 95 %) were 148.52 ± 13.5 and 274.20 ± 40.21 μg/L for 10 and 20 dpf larvae, respectively. The caloric content, glycogen, and lipid concentration were markedly reduced in the 10 dpf larvae exposed to Cu. The malondialdehyde concentration was significantly modified in D. rerio larvae exposed during 48 h to the toxicant. Cu2+ concentrations as low as 16.04 and32.08 μg/L produced biochemical impairment in D. rerio exogenous feeding larvae. Our results warn about harmful effects on the aquatic biota exposed to Cu2+, because its concentrations in polluted waters can be higher than those assayed here. 2015 artículo científico 0188-4999 https://www.redalyc.org/articulo.oa?id=37043336007 https://www.redalyc.org/journal/370/37043336007/ https://www.redalyc.org/journal/370/37043336007/html/ https://www.redalyc.org/journal/370/37043336007/37043336007.epub https://www.redalyc.org/journal/370/37043336007/movil en http://www.redalyc.org/revista.oa?id=370 Revista Internacional de Contaminación Ambiental application/pdf Universidad Nacional Autónoma de México Revista Internacional de Contaminación Ambiental (México) Num.4 Vol.31 |
| title | ACUTE TOXICITY AND SUBLETHAL EFFECTS ON MACROMOLECULES CONCENTRATION, CALORIC CONTENT, AND LIPID PEROXIDATION DURING EXOGENOUS-FEEDING OF Danio rerio LARVAE EXPOSED TO Cu2+ |
| topic | Ciencias de la Tierra Zebrafish biomarkers toxic metals oxidative stress early life stages |
| url | https://www.redalyc.org/articulo.oa?id=37043336007 https://www.redalyc.org/journal/370/37043336007/ https://www.redalyc.org/journal/370/37043336007/html/ https://www.redalyc.org/journal/370/37043336007/37043336007.epub https://www.redalyc.org/journal/370/37043336007/movil |