Saved in:
| Main Author: | |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad del Zulia
2007
|
| Subjects: | |
| Online Access: | https://www.redalyc.org/articulo.oa?id=373062012003 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Table of Contents:
- Apoptotic effect on HeLa Cells produced by Chlamydia trachomatis-LPS Beatriz Millán-Mendoza Hamid Hakimi Adrian Eley Multidisciplinaria (Ciencias Naturales y Exactas) LPS apoptosis Chlamydia trachomatis nuclear supravital fluorescent stain The interaction between the lipopolysaccharide (LPS) of Chlamydia trachomatis and mammalian cells is still largely unknown. Chlamydia trachomatis is an obligate intracellular bacterium responsible for several diseases in humans and animals. In this work, thanks to the isolation of the lipopolysaccharide from two serovars of Chlamydia trachomatis (LGV1-LGV2) and using a nuclear supravital fluorescent stain (Hoechst 33258), it was possible to investigate the apoptotic effect on HeLa cells. This work shows the apoptotic effect on HeLa cells when they were exposed to C. trachomatis-LPS from two serovars at concentrations equal to or higher than 0.5 µg/mL for a period of 48h. and also the lack of cellular response in the absence of C. trachomatis-LPS or in the presence of LPS obtained from other bacteria. Additionally, the use in equal conditions of polymyxin B, known as an inhibitor of bacterial LPS, showed a decrease of the apoptotic effect in such cells indicating that the cellular response observed was produced by C. trachomatis-LPS. These results support the theory that the LPS from C. trachomatis could be responsible for the toxic effect on cervical cells infected by these bacteria. 2007 artículo científico 0075-5222 https://www.redalyc.org/articulo.oa?id=373062012003 en http://www.redalyc.org/revista.oa?id=3730 Kasmera application/pdf Universidad del Zulia Kasmera (República Bolivariana de Venezuela) Num.1 Vol.35