Decrease in Streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults
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| Format: | Artículo científico |
| Langue: | en |
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Centro Nacional de Información de Ciencias Médicas
2019
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| _version_ | 1876464967077593088 |
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| author | Patricio Vildósola Grez |
| author_facet | Patricio Vildósola Grez |
| contents | Decrease in Streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults Patricio Vildósola Grez Eduardo Fernandez Jose Roberto Cury Saad Patricia Palma Fluxá Alain Manuel Chaple Gil Natalia Acuña Zepeda Gustavo Moncada Cortés Medicina composite resins Streptococcus mutans Pit and fissure sealants permanent dental restoration Introduction: The main strategy for the prevention of caries disease is the use of pit and fissure sealants, which are indicated for posterior occlusal surfaces that are difficult to clean and cannot be protected very well by fluoride. This strategy is considered to be very important in caries prevention, especially in patients at high cariogenic risk. Objective: Evaluate whether the count of colony forming units of S. mutans per cm2 on the occlusal surface of posterior permanent teeth changes after applying resin-based sealant. Methods: A study was conducted of 38 patients of both genders aged 18-30 years. The patients were at high cariogenic risk and had an indication of occlusal sealant application on at least one permanent posterior tooth. Two samples were taken of dental biofilm from the same teeth before (group T0) and after (group T1) applying sealant obtained by direct impression by tray technique with trypticase yeast extract, cysteine, sucrose with bacitracin (TYCSB), and agar previously made and solidified. The samples were incubated at 37 ºC for 48 hours. The colony forming units (CFU/cm2) were counted. The results were statistically analyzed with the Wilcoxon test at 95 % confidence. Results: Average expression in CFU/cm2 before applying the sealant (T0) and standard deviation was 13.48 (± 14.2), whereas after applying the sealant (T1) it was 5.37 (± 8.90). There was a statistical difference between the two measures T0 and T1 (p< 0.05). Conclusions: Sealant application on occlusal surfaces of posterior permanent teeth significantly reduces the count of CFU/cm2 of S. mutans. 2019 artículo científico 0034-7507 https://www.redalyc.org/articulo.oa?id=378660742007 https://www.redalyc.org/journal/3786/378660742007/ https://www.redalyc.org/journal/3786/378660742007/html/ https://www.redalyc.org/journal/3786/378660742007/378660742007.epub https://www.redalyc.org/journal/3786/378660742007/movil en http://www.redalyc.org/revista.oa?id=3786 Revista Cubana de Estomatología application/pdf Centro Nacional de Información de Ciencias Médicas Revista Cubana de Estomatología (Cuba) Num.4 Vol.56 |
| format | Artículo científico |
| id | redalyc_378660742007 |
| institution | Redalyc |
| language | en |
| publishDate | 2019 |
| publisher | Centro Nacional de Información de Ciencias Médicas |
| spellingShingle | Decrease in Streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults Patricio Vildósola Grez Medicina composite resins Streptococcus mutans Pit and fissure sealants permanent dental restoration Decrease in Streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults Patricio Vildósola Grez Eduardo Fernandez Jose Roberto Cury Saad Patricia Palma Fluxá Alain Manuel Chaple Gil Natalia Acuña Zepeda Gustavo Moncada Cortés Medicina composite resins Streptococcus mutans Pit and fissure sealants permanent dental restoration Introduction: The main strategy for the prevention of caries disease is the use of pit and fissure sealants, which are indicated for posterior occlusal surfaces that are difficult to clean and cannot be protected very well by fluoride. This strategy is considered to be very important in caries prevention, especially in patients at high cariogenic risk. Objective: Evaluate whether the count of colony forming units of S. mutans per cm2 on the occlusal surface of posterior permanent teeth changes after applying resin-based sealant. Methods: A study was conducted of 38 patients of both genders aged 18-30 years. The patients were at high cariogenic risk and had an indication of occlusal sealant application on at least one permanent posterior tooth. Two samples were taken of dental biofilm from the same teeth before (group T0) and after (group T1) applying sealant obtained by direct impression by tray technique with trypticase yeast extract, cysteine, sucrose with bacitracin (TYCSB), and agar previously made and solidified. The samples were incubated at 37 ºC for 48 hours. The colony forming units (CFU/cm2) were counted. The results were statistically analyzed with the Wilcoxon test at 95 % confidence. Results: Average expression in CFU/cm2 before applying the sealant (T0) and standard deviation was 13.48 (± 14.2), whereas after applying the sealant (T1) it was 5.37 (± 8.90). There was a statistical difference between the two measures T0 and T1 (p< 0.05). Conclusions: Sealant application on occlusal surfaces of posterior permanent teeth significantly reduces the count of CFU/cm2 of S. mutans. 2019 artículo científico 0034-7507 https://www.redalyc.org/articulo.oa?id=378660742007 https://www.redalyc.org/journal/3786/378660742007/ https://www.redalyc.org/journal/3786/378660742007/html/ https://www.redalyc.org/journal/3786/378660742007/378660742007.epub https://www.redalyc.org/journal/3786/378660742007/movil en http://www.redalyc.org/revista.oa?id=3786 Revista Cubana de Estomatología application/pdf Centro Nacional de Información de Ciencias Médicas Revista Cubana de Estomatología (Cuba) Num.4 Vol.56 |
| title | Decrease in Streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults |
| topic | Medicina composite resins Streptococcus mutans Pit and fissure sealants permanent dental restoration |
| url | https://www.redalyc.org/articulo.oa?id=378660742007 https://www.redalyc.org/journal/3786/378660742007/ https://www.redalyc.org/journal/3786/378660742007/html/ https://www.redalyc.org/journal/3786/378660742007/378660742007.epub https://www.redalyc.org/journal/3786/378660742007/movil |