Analysis of virulence genes in Escherichia coli isolated from grated cheese
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| Format: | Artículo científico |
| Language: | en |
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Sociedade Brasileira de Ciência e Tecnologia de Alimentos
2011
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| _version_ | 1876487674091536384 |
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| author | Rodrigo Prado MARTINS |
| author_facet | Rodrigo Prado MARTINS |
| contents | Analysis of virulence genes in Escherichia coli isolated from grated cheese Rodrigo Prado MARTINS Luciano NAKAZATO Valéria DUTRA Domingos da Silva LEITE Agrociencias MT Cuiabá grated cheese virulence genes Escherichia coli This research aimed to verify the presence of virulence genes in strains of Escherichia coli isolated from grated cheese sold in farmers’ markets of Cuiabá-MT, Brazil. Forty samples of this food were submitted for microbiological analysis and 22 (55%) tested positive for E. coli . Next, 64 strains of E. coli were isolated from the positive samples and screened by the polymerase chain reaction (PCR) for the presence of the genes encoding the following virulence factors: stx1 and stx2 (verotoxin types 1 and 2), eae (intimin), lt1 (heat-labile toxin type 1), st1 (heat-stable toxin type 1), cnf1 and cnf2 (cytotoxic necrozing factor types 1 and 2), and cdtB (cytolethal distending toxin). All the isolates were negative for the genes stx1, stx2, eae, lt1, st1, cnf1, and cdtB, and five strains (7.81%) were positive for cnf2 . A low prevalence of E. coli positive for virulence factors associated with the pathogenesis of diarrhoea was observed in this study. However, the presence of CNF-2 producing strains and the possibility of occurrence and scattering of other virulence factors that were not surveyed in the work indicate the risk related to the consumption of grated cheese from farmers’ markets. 2011 artículo científico 0101-2061 https://www.redalyc.org/articulo.oa?id=395940104014 en http://www.redalyc.org/revista.oa?id=3959 Ciência e Tecnologia de Alimentos application/pdf Sociedade Brasileira de Ciência e Tecnologia de Alimentos Ciência e Tecnologia de Alimentos (Brasil) Num.1 Vol.31 |
| format | Artículo científico |
| id | redalyc_395940104014 |
| institution | Redalyc |
| language | en |
| publishDate | 2011 |
| publisher | Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
| spellingShingle | Analysis of virulence genes in Escherichia coli isolated from grated cheese Rodrigo Prado MARTINS Agrociencias MT Cuiabá grated cheese virulence genes Escherichia coli Analysis of virulence genes in Escherichia coli isolated from grated cheese Rodrigo Prado MARTINS Luciano NAKAZATO Valéria DUTRA Domingos da Silva LEITE Agrociencias MT Cuiabá grated cheese virulence genes Escherichia coli This research aimed to verify the presence of virulence genes in strains of Escherichia coli isolated from grated cheese sold in farmers’ markets of Cuiabá-MT, Brazil. Forty samples of this food were submitted for microbiological analysis and 22 (55%) tested positive for E. coli . Next, 64 strains of E. coli were isolated from the positive samples and screened by the polymerase chain reaction (PCR) for the presence of the genes encoding the following virulence factors: stx1 and stx2 (verotoxin types 1 and 2), eae (intimin), lt1 (heat-labile toxin type 1), st1 (heat-stable toxin type 1), cnf1 and cnf2 (cytotoxic necrozing factor types 1 and 2), and cdtB (cytolethal distending toxin). All the isolates were negative for the genes stx1, stx2, eae, lt1, st1, cnf1, and cdtB, and five strains (7.81%) were positive for cnf2 . A low prevalence of E. coli positive for virulence factors associated with the pathogenesis of diarrhoea was observed in this study. However, the presence of CNF-2 producing strains and the possibility of occurrence and scattering of other virulence factors that were not surveyed in the work indicate the risk related to the consumption of grated cheese from farmers’ markets. 2011 artículo científico 0101-2061 https://www.redalyc.org/articulo.oa?id=395940104014 en http://www.redalyc.org/revista.oa?id=3959 Ciência e Tecnologia de Alimentos application/pdf Sociedade Brasileira de Ciência e Tecnologia de Alimentos Ciência e Tecnologia de Alimentos (Brasil) Num.1 Vol.31 |
| title | Analysis of virulence genes in Escherichia coli isolated from grated cheese |
| topic | Agrociencias MT Cuiabá grated cheese virulence genes Escherichia coli |
| url | https://www.redalyc.org/articulo.oa?id=395940104014 |