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| Autore principale: | |
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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos
2011
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| Soggetti: | |
| Accesso online: | https://www.redalyc.org/articulo.oa?id=395940111019 |
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Sommario:
- Standardization of a multiplex PCR for the identification of coagulase-positive Staphylococcus Eliezer Avila GANDRA Maria Aparecida FERNANDEZ Jorge Adolfo SILVA Wladimir Padilha da SILVA Agrociencias PCR aureus hyicus nuc gene intermedius The enterotoxigenic species Staphylococcus aureus, S. hyicus and S. intermedius show very similar characteristics, making their identification through conventional microbiological methods difficult. This study aimed at the development of a Multiplex PCR (mPCR) for the identification of S. aureus, S. intermedius and S. hyicus using the nuc gene as the target sequence. The results obtained suggest that the set of primers used was specific for the three species of Staphylococcus evaluate with a detection limit of 102 CFU.mL–1. 2011 artículo científico 0101-2061 https://www.redalyc.org/articulo.oa?id=395940111019 en http://www.redalyc.org/revista.oa?id=3959 Ciência e Tecnologia de Alimentos application/pdf Sociedade Brasileira de Ciência e Tecnologia de Alimentos Ciência e Tecnologia de Alimentos (Brasil) Num.4 Vol.31