Gene expression of endothelin receptors in replaced rheumatic mitral stenotic valves
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| Natura: | Artículo científico |
| Lingua: | en |
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Sociedade Brasileira de Cirurgia Cardiovascular
2012
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| author | Sydney Correia Leão |
| author_facet | Sydney Correia Leão |
| contents | Gene expression of endothelin receptors in replaced rheumatic mitral stenotic valves Sydney Correia Leão Fernanda Maria Silveira Souto Ricardo Vieira da Costa Thaisa de Fatima Almeida Rocha Yolanda Galindo Pacheco Tania Maria de Andrade Rodrigues Medicina Endothelins Rheumatic fever Mitral valve stenosis Objectives: Rheumatic fever is a highly prevalent disease in Brazil, and it poses a major public health problem. It is the leading cause of acquired heart disease in childhood and adolescence. The aim of this study was to evaluate the gene expression of ET-3 and its receptors, in replaced rheumatic mitral valves. Methods: We studied the gene expression of endothelin-3 (ET-3) and its receptors, endothelin receptor A and endothelin receptor B (ETr-A and ETr-B), in the rheumatic mitral valves of 17 patients who underwent valve replacement surgery. The samples also underwent a histological analysis. Results: Our data showed that almost all patients, regardless of individual characteristics such as gender or age, expressed the endothelin receptor genes, but did not express the genes for ET-3. In quantitative analysis, the ETr-A/GAPDH mean ratio was 33.04 ± 18.09%; while the ETr-B/GAPDH mean ratio was 114.58 ± 42.30%. Regarding histopathological individual features, the frequency of fibrosis is 100%, 88.23% of mononuclear infiltrate, 52.94% of neovascularization, 58.82% of calcification and absence of ossification. Conclusion: The presence of receptors ETr-A and ETr-B in rheumatic mitral valves suggests its interaction with the system of circulating endothelins, particularly ETr-B (known for acting in the removal of excess endothelin) detected in a greater proportion, which could explain the lack of expression of endothelin in rheumatic mitral valve, process to be elucidated. 2012 artículo científico 0102-7638 https://www.redalyc.org/articulo.oa?id=398941887008 en http://www.redalyc.org/revista.oa?id=3989 Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery application/pdf Sociedade Brasileira de Cirurgia Cardiovascular Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery (Brasil) Num.4 Vol.27 |
| format | Artículo científico |
| id | redalyc_398941887008 |
| institution | Redalyc |
| language | en |
| publishDate | 2012 |
| publisher | Sociedade Brasileira de Cirurgia Cardiovascular |
| spellingShingle | Gene expression of endothelin receptors in replaced rheumatic mitral stenotic valves Sydney Correia Leão Medicina Endothelins Rheumatic fever Mitral valve stenosis Gene expression of endothelin receptors in replaced rheumatic mitral stenotic valves Sydney Correia Leão Fernanda Maria Silveira Souto Ricardo Vieira da Costa Thaisa de Fatima Almeida Rocha Yolanda Galindo Pacheco Tania Maria de Andrade Rodrigues Medicina Endothelins Rheumatic fever Mitral valve stenosis Objectives: Rheumatic fever is a highly prevalent disease in Brazil, and it poses a major public health problem. It is the leading cause of acquired heart disease in childhood and adolescence. The aim of this study was to evaluate the gene expression of ET-3 and its receptors, in replaced rheumatic mitral valves. Methods: We studied the gene expression of endothelin-3 (ET-3) and its receptors, endothelin receptor A and endothelin receptor B (ETr-A and ETr-B), in the rheumatic mitral valves of 17 patients who underwent valve replacement surgery. The samples also underwent a histological analysis. Results: Our data showed that almost all patients, regardless of individual characteristics such as gender or age, expressed the endothelin receptor genes, but did not express the genes for ET-3. In quantitative analysis, the ETr-A/GAPDH mean ratio was 33.04 ± 18.09%; while the ETr-B/GAPDH mean ratio was 114.58 ± 42.30%. Regarding histopathological individual features, the frequency of fibrosis is 100%, 88.23% of mononuclear infiltrate, 52.94% of neovascularization, 58.82% of calcification and absence of ossification. Conclusion: The presence of receptors ETr-A and ETr-B in rheumatic mitral valves suggests its interaction with the system of circulating endothelins, particularly ETr-B (known for acting in the removal of excess endothelin) detected in a greater proportion, which could explain the lack of expression of endothelin in rheumatic mitral valve, process to be elucidated. 2012 artículo científico 0102-7638 https://www.redalyc.org/articulo.oa?id=398941887008 en http://www.redalyc.org/revista.oa?id=3989 Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery application/pdf Sociedade Brasileira de Cirurgia Cardiovascular Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery (Brasil) Num.4 Vol.27 |
| title | Gene expression of endothelin receptors in replaced rheumatic mitral stenotic valves |
| topic | Medicina Endothelins Rheumatic fever Mitral valve stenosis |
| url | https://www.redalyc.org/articulo.oa?id=398941887008 |