Comparação da solução de histidina-triptofano-alfacetoglutarato com histidina-triptofano-glutamato como agentes cardioplégicos em corações isolados de ratos: estudo imuno-histoquímico
Fuente:
Redalyc
Guardado en:
| Autor principal: | |
|---|---|
| Formato: | Artículo científico |
| Lenguaje: | pt |
| Publicado: |
Sociedade Brasileira de Cirurgia Cardiovascular
2014
|
| Materias: | |
| Acceso en línea: | |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| _version_ | 1876446908568829952 |
|---|---|
| author | Marcos Aurélio Barboza de Oliveira |
| author_facet | Marcos Aurélio Barboza de Oliveira |
| contents | Comparação da solução de histidina-triptofano-alfacetoglutarato com histidina-triptofano-glutamato como agentes cardioplégicos em corações isolados de ratos: estudo imuno-histoquímico Marcos Aurélio Barboza de Oliveira Lívia Carvalho Ferreira Débora Aparecida Pires de Campos Zuccari Antônio Carlos Brandi Carlos Alberto dos Santos Paulo Henrique Husseni Botelho Orlando Petrucci Domingo Marcolino Braile Medicina Heart Induced Heart Arrest Myocardial Ischemia Introduction: Cardiac arrest during heart surgery is a com - mon procedure and allows the surgeon to perform surgical pro - cedures in an environment free of blood and movement. Using a model of isolated rat heart, the authors compare a new car - dioplegic solution containing histidine-tryptophan-glutamate (group 2) with the histidine-tryptophan-alphacetoglutarate (group 1) routinely used by some cardiac surgeons. Objective: To assess caspase, IL-8 and KI-67 in isolated rat hearts using i mmunohistochemistry. Methods: 20 Wistar male rats were anesthetized and heparinized. The chest was opened, cardioctomy was performed and 40 ml/kg of the appropriate cardioplegic solution was infused. The hearts were kept for 2 hours at 4°C in the same solution, and thereafter, placed in the Langendorff apparatus for 30 minutes with Ringer-Locke solution. Immunohistochemistry analysis of caspase, IL-8, and KI-67 were performed. Results: The concentration of caspase was lower in group 2 and Ki-67 was higher in group 2, both P <0.05. There was no statistical difference between the values of IL-8 between the groups. Conclusion: Histidine-tryptophan-glutamate solution was better than histidine-tryptophan-alphacetoglutarate solution because it reduced caspase (apoptosis), increased KI-67 (cell proliferation), and showed no difference in IL-8 levels compared to group 1. This suggests that the histidine-tryptophan-glutamate solution was more efficient than the histidine-tryptophan- alphacetoglutarate for the preservation of hearts of rat cardiomyocytes. 2014 artículo científico 0102-7638 https://www.redalyc.org/articulo.oa?id=398941892015 pt http://www.redalyc.org/revista.oa?id=3989 Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery application/pdf Sociedade Brasileira de Cirurgia Cardiovascular Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery (Brasil) Num.1 Vol.29 |
| format | Artículo científico |
| id | redalyc_398941892015 |
| institution | Redalyc |
| language | pt |
| publishDate | 2014 |
| publisher | Sociedade Brasileira de Cirurgia Cardiovascular |
| spellingShingle | Comparação da solução de histidina-triptofano-alfacetoglutarato com histidina-triptofano-glutamato como agentes cardioplégicos em corações isolados de ratos: estudo imuno-histoquímico Marcos Aurélio Barboza de Oliveira Medicina Heart Induced Heart Arrest Myocardial Ischemia Comparação da solução de histidina-triptofano-alfacetoglutarato com histidina-triptofano-glutamato como agentes cardioplégicos em corações isolados de ratos: estudo imuno-histoquímico Marcos Aurélio Barboza de Oliveira Lívia Carvalho Ferreira Débora Aparecida Pires de Campos Zuccari Antônio Carlos Brandi Carlos Alberto dos Santos Paulo Henrique Husseni Botelho Orlando Petrucci Domingo Marcolino Braile Medicina Heart Induced Heart Arrest Myocardial Ischemia Introduction: Cardiac arrest during heart surgery is a com - mon procedure and allows the surgeon to perform surgical pro - cedures in an environment free of blood and movement. Using a model of isolated rat heart, the authors compare a new car - dioplegic solution containing histidine-tryptophan-glutamate (group 2) with the histidine-tryptophan-alphacetoglutarate (group 1) routinely used by some cardiac surgeons. Objective: To assess caspase, IL-8 and KI-67 in isolated rat hearts using i mmunohistochemistry. Methods: 20 Wistar male rats were anesthetized and heparinized. The chest was opened, cardioctomy was performed and 40 ml/kg of the appropriate cardioplegic solution was infused. The hearts were kept for 2 hours at 4°C in the same solution, and thereafter, placed in the Langendorff apparatus for 30 minutes with Ringer-Locke solution. Immunohistochemistry analysis of caspase, IL-8, and KI-67 were performed. Results: The concentration of caspase was lower in group 2 and Ki-67 was higher in group 2, both P <0.05. There was no statistical difference between the values of IL-8 between the groups. Conclusion: Histidine-tryptophan-glutamate solution was better than histidine-tryptophan-alphacetoglutarate solution because it reduced caspase (apoptosis), increased KI-67 (cell proliferation), and showed no difference in IL-8 levels compared to group 1. This suggests that the histidine-tryptophan-glutamate solution was more efficient than the histidine-tryptophan- alphacetoglutarate for the preservation of hearts of rat cardiomyocytes. 2014 artículo científico 0102-7638 https://www.redalyc.org/articulo.oa?id=398941892015 pt http://www.redalyc.org/revista.oa?id=3989 Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery application/pdf Sociedade Brasileira de Cirurgia Cardiovascular Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery (Brasil) Num.1 Vol.29 |
| title | Comparação da solução de histidina-triptofano-alfacetoglutarato com histidina-triptofano-glutamato como agentes cardioplégicos em corações isolados de ratos: estudo imuno-histoquímico |
| topic | Medicina Heart Induced Heart Arrest Myocardial Ischemia |
| url | https://www.redalyc.org/articulo.oa?id=398941892015 |