Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti-CD34 antibody
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| Format: | Artículo científico |
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Sociedade Brasileira de Cirurgia Cardiovascular
2015
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| _version_ | 1876423053454344192 |
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| author | Chun-Li Song |
| author_facet | Chun-Li Song |
| contents | Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti-CD34 antibody Chun-Li Song Qian Li Yun-Peng Yu Guan Wang Jin-Peng Wang Yang Lu Ji-Chang Zhang Hong-Ying Diao Jian-Gen Liu Yi-Hang Liu Jia Liu Ying Li Dan Cai Bin Liu Medicina Drug CD34 Antigens Eluting Stents Coronary Restenosis Introduction: Intravascular coronary stenting has been used in the treatment of coronary artery disease (CAD), with a major limitation of in-stent restenosis (ISR). The 316 stainless steel has been widely used for coronary stents. In this study, we developed a novel coating method to reduce ISR by simultane - ously coating vascular endothelial growth factor (VEGF) and anti-CD34 antibody on 316L stainless steel. Methods: Round 316L stainless steel sheets in the D-H group were polymerized with compounds generated from condensa - tion reaction of dopamine and heparin using N-(3-dimethylami - nopropyl)-N’-ethylcarbodiimide (EDC) and N-hydroxysuccin - imide (NHS). Sixteen sheets from the D-H group were further immersed into 1ug/ml VEGF 165 and 3mg/ml heparin sodium one after another for 10 times, and named as the D-(H-V) 10 group. Eight sheets from the D-(H-V) 10 group were coated with an - ti-CD34 antibody and termed as the D-(H-V) 10 -A group. Immu - nofluorescence assay and ELISA were used to evaluate wheth - er the 316L stainless steel disks were successfully coated with VEGF and anti-CD34 antibody. Results: The results of immunofluorescence assay and ELI - SA showed that VEGF could be detected in the D-(H-V) 10 and D-(H-V) 10 -A group, suggesting the steel sheets were successful - ly covered with VEGF. Anti-CD34 antibody could only be ob - served in the D-(H-V) 10 -A group, which was the only group coat - ed with CD34 antibody. Both results suggested that the 316L stainless steel sheets were successfully coated with VEGF and anti-CD34 antibody. Conclusion: Our study developed a method to simultaneous - ly coat VEGF and anti-CD34 antibody to stainless metal steel. This research serves as a fundamental role for a novel coating strategy. 2015 artículo científico 0102-7638 https://www.redalyc.org/articulo.oa?id=398941897005 pt http://www.redalyc.org/revista.oa?id=3989 Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery application/pdf Sociedade Brasileira de Cirurgia Cardiovascular Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery (Brasil) Num.2 Vol.30 |
| format | Artículo científico |
| id | redalyc_398941897005 |
| institution | Redalyc |
| language | pt |
| publishDate | 2015 |
| publisher | Sociedade Brasileira de Cirurgia Cardiovascular |
| spellingShingle | Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti-CD34 antibody Chun-Li Song Medicina Drug CD34 Antigens Eluting Stents Coronary Restenosis Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti-CD34 antibody Chun-Li Song Qian Li Yun-Peng Yu Guan Wang Jin-Peng Wang Yang Lu Ji-Chang Zhang Hong-Ying Diao Jian-Gen Liu Yi-Hang Liu Jia Liu Ying Li Dan Cai Bin Liu Medicina Drug CD34 Antigens Eluting Stents Coronary Restenosis Introduction: Intravascular coronary stenting has been used in the treatment of coronary artery disease (CAD), with a major limitation of in-stent restenosis (ISR). The 316 stainless steel has been widely used for coronary stents. In this study, we developed a novel coating method to reduce ISR by simultane - ously coating vascular endothelial growth factor (VEGF) and anti-CD34 antibody on 316L stainless steel. Methods: Round 316L stainless steel sheets in the D-H group were polymerized with compounds generated from condensa - tion reaction of dopamine and heparin using N-(3-dimethylami - nopropyl)-N’-ethylcarbodiimide (EDC) and N-hydroxysuccin - imide (NHS). Sixteen sheets from the D-H group were further immersed into 1ug/ml VEGF 165 and 3mg/ml heparin sodium one after another for 10 times, and named as the D-(H-V) 10 group. Eight sheets from the D-(H-V) 10 group were coated with an - ti-CD34 antibody and termed as the D-(H-V) 10 -A group. Immu - nofluorescence assay and ELISA were used to evaluate wheth - er the 316L stainless steel disks were successfully coated with VEGF and anti-CD34 antibody. Results: The results of immunofluorescence assay and ELI - SA showed that VEGF could be detected in the D-(H-V) 10 and D-(H-V) 10 -A group, suggesting the steel sheets were successful - ly covered with VEGF. Anti-CD34 antibody could only be ob - served in the D-(H-V) 10 -A group, which was the only group coat - ed with CD34 antibody. Both results suggested that the 316L stainless steel sheets were successfully coated with VEGF and anti-CD34 antibody. Conclusion: Our study developed a method to simultaneous - ly coat VEGF and anti-CD34 antibody to stainless metal steel. This research serves as a fundamental role for a novel coating strategy. 2015 artículo científico 0102-7638 https://www.redalyc.org/articulo.oa?id=398941897005 pt http://www.redalyc.org/revista.oa?id=3989 Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery application/pdf Sociedade Brasileira de Cirurgia Cardiovascular Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery (Brasil) Num.2 Vol.30 |
| title | Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti-CD34 antibody |
| topic | Medicina Drug CD34 Antigens Eluting Stents Coronary Restenosis |
| url | https://www.redalyc.org/articulo.oa?id=398941897005 |