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| Format: | Artículo científico |
| Language: | en |
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Sociedade Brasileira de Cirurgia Cardiovascular
2023
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| Online Access: | https://www.redalyc.org/articulo.oa?id=398975374004 https://www.redalyc.org/journal/3989/398975374004/ https://www.redalyc.org/journal/3989/398975374004/html/ https://www.redalyc.org/journal/3989/398975374004/398975374004.epub https://www.redalyc.org/journal/3989/398975374004/movil |
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Table of Contents:
- Protective Effects of Fuziline on Dobutamine-Induced Heart Damage in Mice Yasemin Hacanli Mehmet Salih Aydin Ezhar Korkmaz Ersöz Nazim Kankiliç İsmail Koyuncu Muhammet Emin Güldür Ebru Temiz Reşat Dikme Kadir Eği Yusuf Çakmak Mahmut Padak Medicina Introduction: Fuziline is one of the many antioxidants currently being tested to treat cardiac damage. In our study, histopathological and biochemical effects of fuziline were investigated in mice with dobutamine-induced heart damage in vitro. Methods: Thirty-two adult male BALB/c mice, average weight of 18-20 g, were randomly divided into four groups — Group 1 (sham, n=8), Group 2 (control, dobutamine, n=8), Group 3 (treatment 1, dobutamine + fuziline, n=8), and Group 4 (treatment 2, fuziline, n=8). Biochemical parameters and total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) values were measured. Interleukin 1 beta (IL-1β), NLR family, pyrin domain containing protein 3 (NLRP3), 8-hydroxy-deoxyguanosine (8-OHDG), gasdermin D (GSDMD), and galectin 3 (GAL-3) levels were analyzed by enzyme-linked immunosorbent assay method, and histopathological examination of heart tissues was performed. Results: When dobutamine + fuziline and fuziline groups were compared, troponin-I (P<0.05), NLRP3 (P<0.001), GSDMD (P<0.001), 8-OHDG (P<0.001), IL-1β (P<0.001), and GAL-3 (P<0.05) were found to be statistically significant. TOS level was the highest in the dobutamine group (P<0.001) and TAS level was the highest in the fuziline group (P<0.001). OSI level was statistically significant between the groups (P<0.001). In histopathological examination, focal necrosis areas were smaller in the dobutamine + fuziline group than in the dobutamine group, and cardiac myocytes were better preserved. Conclusion: Fuziline markedly reduced cardiac damage and pyroptosis in mice with dobutamine-induced heart damage by lowering the levels of GSDMD, 8-OHDG, IL-1β, and GAL-3. It also prevented necrosis of cardiac myocytes in histopathological evaluation. 2023 artículo científico 0102-7638 https://www.redalyc.org/articulo.oa?id=398975374004 https://www.redalyc.org/journal/3989/398975374004/ https://www.redalyc.org/journal/3989/398975374004/html/ https://www.redalyc.org/journal/3989/398975374004/398975374004.epub https://www.redalyc.org/journal/3989/398975374004/movil 10.21470/1678-9741-2022-0251 en http://www.redalyc.org/revista.oa?id=3989 Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery application/pdf Sociedade Brasileira de Cirurgia Cardiovascular Revista Brasileira de Cirurgia Cardiovascular/Brazilian Journal of Cardiovascular Surgery (Brasil) Num.4 Vol.38