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| Format: | Artículo científico |
| Langue: | pt |
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Sociedade de Pediatria de São Paulo
2015
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| Accès en ligne: | https://www.redalyc.org/articulo.oa?id=406039569003 |
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Table des matières:
- Aplicação de teste molecular para detecção de adenovírus em pacientes pediátricos distintos Diane Puerari Clarice Camargo Sandra Gratura Aripuanã Sakurada Aranha Watanabe Celso Granato Nancy Cristina Junqueira Bellei Medicina Respiratory viral infection Adenovirus and polymerase chain reaction OBJECTIVE:Adenoviruses play an important role in the etiology of severe acute lower respiratory infection, especially in young children. The aim of the present study was to evaluate the Human Adenovirus (HAdV) detection by different methods (Direct Fluorescence Assay DFA and Nested Polymerase Chain Reaction nested PCR), among samples collected from different groups of pediatric patients.METHODS:Collection of samples was made in children with congenital heart disease (CHD 123 nasal aspirates collected in the years of 2005, 2007 and 2008) and in community children (CC 165 nasal aspirates collected in 2008). Children were eligible if they presented acute respiratory infection (ARI) of probable viral etiology, within up to 7 days of symptoms' onset. All studied samples were evaluated by DFA and nested PCR assay.RESULTS:Of the 290 samples included during the study period, 43 (14.8%) were positive on at least one test: 17/165 (10.3%) of the CC and 26/125 (20.8%) of the CHD children. The nested PCR detection rates in the community children were 15/165 (9.1%), and for children with CHD, 24/125 (19.2%). Molecular method showed higher detection rates when compared to the DFA test (p<0.001). Univariate analysis showed that children with congenital heart disease presented a significantly higher chance for acquiring the HAdV (Odds Ratio 2.3; 95% CI: 1.18-4.43).CONCLUSIONS:Based on data obtained in the present evaluation, we suggest that a routine surveillance should be performed in high risk patients by molecular methods, thus improving diagnostic flow and efficiency. 2015 artículo científico 0103-0582 https://www.redalyc.org/articulo.oa?id=406039569003 pt http://www.redalyc.org/revista.oa?id=4060 Revista Paulista de Pediatria application/pdf Sociedade de Pediatria de São Paulo Revista Paulista de Pediatria (Brasil) Num.2 Vol.33