Isolation of Escherichia coli and Klebsiella pneumoniae strains producing extended-spectrum ß-lactamases from dog urine of the Metropolitan Area of the Aburrá Valley (Antioquia, Colombia)
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| Format: | Artículo científico |
| Langue: | en |
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Universidad Nacional de Colombia
2022
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| _version_ | 1876461941258452992 |
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| author | A. M. Ochoa |
| author_facet | A. M. Ochoa |
| contents | Isolation of Escherichia coli and Klebsiella pneumoniae strains producing extended-spectrum ß-lactamases from dog urine of the Metropolitan Area of the Aburrá Valley (Antioquia, Colombia) A. M. Ochoa M. I. García A.V. Cienfuegos L. Vásquez-Jaramillo Veterinaria perros salud pública enterobacterales Resistencia a betalactámicos infección del tracto urinario Escherichia coli and Klebsiella pneumoniae are the most common pathogens causing urinary tract infections in humans and animals. Close contact between humans and companion animals can facilitate the spread of multidrug resistant pathogens between both species. The objective of the research was to characterize extended-spectrum ß-lactamases (ESBL) -producing E. coli and K. pneumoniae isolated from dogs with urinary tract infections in the metropolitan area of Valle del Aburrá (Antioquia, Colombia). Three-hundred seventy-one urine samples collected from March 2018 to March 2019 in a veterinary clinical laboratory were analyzed. E. coli and K. pneumoniae isolates were detected in chromogenic agar and identified by biochemical tests. Susceptibility testing was performed by disc diffusion and ESBL production was evaluated by the double disk test in all isolates. MIC determination of ESBL-positive isolates were performed on the automated VITEK®2 system. Multiple PCR was used for the detection of CTX-M beta-lactamases (group 1, 2, 9 and 8/25), SHV, TEM, and AmpC of plasmid origin in ESBL-positive isolates. In total 22 out 371 isolates were positive for ESBL production by double disc test, 11 E. coli (ESBL-Ec) and 11 K. pneumoniae (ESBL-Kp). The multiple PCR detected CTX-M group 1 in the 22 ESBL-positive isolates. Multi-drug resistance was observed in all ESBL-producing isolates. In conclusion, a high frequency of antibiotic multi-resistance was found in ESBL-Ec and ESBL-Kp. The main ESBL detected was CTX-M group 1, which also prevails in human isolates. 2022 artículo científico 0120-2952 https://www.redalyc.org/articulo.oa?id=407674151003 https://www.redalyc.org/journal/4076/407674151003/ https://www.redalyc.org/journal/4076/407674151003/html/ https://www.redalyc.org/journal/4076/407674151003/407674151003.epub https://www.redalyc.org/journal/4076/407674151003/movil 10.15446/rfmvz.v69n3.103805 en http://www.redalyc.org/revista.oa?id=4076 Revista de la Facultad de Medicina Veterinaria y de Zootecnia application/pdf Universidad Nacional de Colombia Revista de la Facultad de Medicina Veterinaria y de Zootecnia (Colombia) Num.3 Vol.69 |
| format | Artículo científico |
| id | redalyc_407674151003 |
| institution | Redalyc |
| language | en |
| publishDate | 2022 |
| publisher | Universidad Nacional de Colombia |
| spellingShingle | Isolation of Escherichia coli and Klebsiella pneumoniae strains producing extended-spectrum ß-lactamases from dog urine of the Metropolitan Area of the Aburrá Valley (Antioquia, Colombia) A. M. Ochoa Veterinaria perros salud pública enterobacterales Resistencia a betalactámicos infección del tracto urinario Isolation of Escherichia coli and Klebsiella pneumoniae strains producing extended-spectrum ß-lactamases from dog urine of the Metropolitan Area of the Aburrá Valley (Antioquia, Colombia) A. M. Ochoa M. I. García A.V. Cienfuegos L. Vásquez-Jaramillo Veterinaria perros salud pública enterobacterales Resistencia a betalactámicos infección del tracto urinario Escherichia coli and Klebsiella pneumoniae are the most common pathogens causing urinary tract infections in humans and animals. Close contact between humans and companion animals can facilitate the spread of multidrug resistant pathogens between both species. The objective of the research was to characterize extended-spectrum ß-lactamases (ESBL) -producing E. coli and K. pneumoniae isolated from dogs with urinary tract infections in the metropolitan area of Valle del Aburrá (Antioquia, Colombia). Three-hundred seventy-one urine samples collected from March 2018 to March 2019 in a veterinary clinical laboratory were analyzed. E. coli and K. pneumoniae isolates were detected in chromogenic agar and identified by biochemical tests. Susceptibility testing was performed by disc diffusion and ESBL production was evaluated by the double disk test in all isolates. MIC determination of ESBL-positive isolates were performed on the automated VITEK®2 system. Multiple PCR was used for the detection of CTX-M beta-lactamases (group 1, 2, 9 and 8/25), SHV, TEM, and AmpC of plasmid origin in ESBL-positive isolates. In total 22 out 371 isolates were positive for ESBL production by double disc test, 11 E. coli (ESBL-Ec) and 11 K. pneumoniae (ESBL-Kp). The multiple PCR detected CTX-M group 1 in the 22 ESBL-positive isolates. Multi-drug resistance was observed in all ESBL-producing isolates. In conclusion, a high frequency of antibiotic multi-resistance was found in ESBL-Ec and ESBL-Kp. The main ESBL detected was CTX-M group 1, which also prevails in human isolates. 2022 artículo científico 0120-2952 https://www.redalyc.org/articulo.oa?id=407674151003 https://www.redalyc.org/journal/4076/407674151003/ https://www.redalyc.org/journal/4076/407674151003/html/ https://www.redalyc.org/journal/4076/407674151003/407674151003.epub https://www.redalyc.org/journal/4076/407674151003/movil 10.15446/rfmvz.v69n3.103805 en http://www.redalyc.org/revista.oa?id=4076 Revista de la Facultad de Medicina Veterinaria y de Zootecnia application/pdf Universidad Nacional de Colombia Revista de la Facultad de Medicina Veterinaria y de Zootecnia (Colombia) Num.3 Vol.69 |
| title | Isolation of Escherichia coli and Klebsiella pneumoniae strains producing extended-spectrum ß-lactamases from dog urine of the Metropolitan Area of the Aburrá Valley (Antioquia, Colombia) |
| topic | Veterinaria perros salud pública enterobacterales Resistencia a betalactámicos infección del tracto urinario |
| url | https://www.redalyc.org/articulo.oa?id=407674151003 https://www.redalyc.org/journal/4076/407674151003/ https://www.redalyc.org/journal/4076/407674151003/html/ https://www.redalyc.org/journal/4076/407674151003/407674151003.epub https://www.redalyc.org/journal/4076/407674151003/movil |