Effect of porcine reproductive and respiratory syndrome (PRRS) virus on porcine monocyte-derived dendritic cells
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Universidad Nacional Autónoma de México
2009
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| _version_ | 1876441251632381952 |
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| author | Lilian Flores-Mendoza |
| author_facet | Lilian Flores-Mendoza |
| contents | Effect of porcine reproductive and respiratory syndrome (PRRS) virus on porcine monocyte-derived dendritic cells Lilian Flores-Mendoza Erika Silva-Campa Mónica Reséndiz Verónica Mata-Haro Fernando A. Osorio Jesús Hernández Multidisciplinaria (Ciencias Naturales y Exactas) II RT MHC PCR CD80 Dendritic cells (DC) are considered the most important antigen presenting cells of the immune system. Its anatomical location (skin, mucosa and peripheral blood), the expression of receptors to recognize pathogens, the expression of co-stimulatory molecules (CD80/86), the major histocompatibility complex (MHC) class I and II, and the production of cytokines (such as IFN-¿, IL-10, IL-12) confers to these cells the characteristic to regulate innate and adaptive immune responses. The objective of this work was to evaluate the effects of the porcine reproductive and respiratory virus (PRRS) in mature DC. DC were generated from blood monocytes using IL-4 and GM-CSF and were stimulated with lipopolysaccharide (LPS) to induce their maturation. The results show that the expression of CD14 and CD172a molecules in infected DC was not affected, while MHC II and CD80/86 expression was diminished. This decrease seems to affect the allogenic proliferation of lymphocytes stimulated with infected DC. On the other hand, the virus increases mRNA expression of IL-10 and TNF-¿, and diminishes that for IL-1B and IL-6. The results obtained could explain, in part, the immunophatology of the disease. 2009 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42311566005 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.1 Vol.40 |
| format | Artículo científico |
| id | redalyc_42311566005 |
| institution | Redalyc |
| language | en |
| publishDate | 2009 |
| publisher | Universidad Nacional Autónoma de México |
| spellingShingle | Effect of porcine reproductive and respiratory syndrome (PRRS) virus on porcine monocyte-derived dendritic cells Lilian Flores-Mendoza Multidisciplinaria (Ciencias Naturales y Exactas) II RT MHC PCR CD80 Effect of porcine reproductive and respiratory syndrome (PRRS) virus on porcine monocyte-derived dendritic cells Lilian Flores-Mendoza Erika Silva-Campa Mónica Reséndiz Verónica Mata-Haro Fernando A. Osorio Jesús Hernández Multidisciplinaria (Ciencias Naturales y Exactas) II RT MHC PCR CD80 Dendritic cells (DC) are considered the most important antigen presenting cells of the immune system. Its anatomical location (skin, mucosa and peripheral blood), the expression of receptors to recognize pathogens, the expression of co-stimulatory molecules (CD80/86), the major histocompatibility complex (MHC) class I and II, and the production of cytokines (such as IFN-¿, IL-10, IL-12) confers to these cells the characteristic to regulate innate and adaptive immune responses. The objective of this work was to evaluate the effects of the porcine reproductive and respiratory virus (PRRS) in mature DC. DC were generated from blood monocytes using IL-4 and GM-CSF and were stimulated with lipopolysaccharide (LPS) to induce their maturation. The results show that the expression of CD14 and CD172a molecules in infected DC was not affected, while MHC II and CD80/86 expression was diminished. This decrease seems to affect the allogenic proliferation of lymphocytes stimulated with infected DC. On the other hand, the virus increases mRNA expression of IL-10 and TNF-¿, and diminishes that for IL-1B and IL-6. The results obtained could explain, in part, the immunophatology of the disease. 2009 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42311566005 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.1 Vol.40 |
| title | Effect of porcine reproductive and respiratory syndrome (PRRS) virus on porcine monocyte-derived dendritic cells |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) II RT MHC PCR CD80 |
| url | https://www.redalyc.org/articulo.oa?id=42311566005 |