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| Formato: | Artículo científico |
| Lenguaje: | es |
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Universidad Nacional Autónoma de México
2010
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| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=42316024006 |
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- Neospora caninum: Detección de ADN en sangre durante la primera gestación de vaquillas infectadas naturalmente Omar Iván Santana Carlos Cruz-Vázquez Leticia Medina-Esparza Miguel Ramos Parra Ciro Castellanos Morales Daniel Quezada Gallardo Multidisciplinaria (Ciencias Naturales y Exactas) DNA PCR blood Mexico dairy herd The aim of the study was to detect the DNA presence of N. caninum in naturally infected animals, at two moments of their first gestation and at parturition, as well as to record the presentation of abortions. Twenty females between 12 to 14 months of age, seropositive to ELISA test, were selected from a dairy farm with presence of this parasitosis. The females were artificially inseminated and blood samples were taken in the first and second third of gestation and during parturition; DNA was isolated and it was analyzed by a single tube nested PCR with specific primers. In the sampling corresponding to the first third of gestation, 7/20 positive cases were observed (35%), in the second 15/20 (75%) and during parturition 10/20 positive cases (50%). From the total of the animals included in this study, three stayed negative to the test in the three samplings (15%), four were always positive (20%), eight were positive in the second sampling but negative in first (40%) and five were positive in the second and negative in first and the third sampling (25%). All animals remained seropositive during the study; four aborted in the last third of gestation. All the live born calves were seropositive to N. caninum. 2010 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42316024006 es http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.2 Vol.41