Identificación de Mycoplasma spp en leche bovina con una prueba de inmunoperoxidasa
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidad Nacional Autónoma de México
2004
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| _version_ | 1876458027421270016 |
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| author | Fidel Infante Martínez |
| author_facet | Fidel Infante Martínez |
| contents | Identificación de Mycoplasma spp en leche bovina con una prueba de inmunoperoxidasa Fidel Infante Martínez Gerardo H. Flores Gutiérrez Javier Lozoya Chávez Multidisciplinaria (Ciencias Naturales y Exactas) mycoplasma bovine milk immunoperoxidase assay immunofluorescence assay The objective of this study was to implement an immunoperoxidase assay for the identifi cation of Mycoplasmaspp from bovine milk. Eighty samples of naturally infected milk, with different Mycoplasma spp,were analyzed; the immunofl uorescence assay was used as a gold standard. The sensitivity of the immunoperoxidaseassay was six colony-forming units, with no cross-reactions between the antisera, with 100%epidemiological sensitivity and specifi city, and predictive values; there were no statistical differences (P >0.05) between both tests. The reproducibility of the immunoperoxidase assay was high, obtaining identicalresults four times. Furthermore, it was carried out in less time than the immunofl uorescence assay,requiring only a light microscope to read the results and fewer antisera and conjugates. In this study, theimmunoperoxidase assay proved to have similar diagnostic value to the immunofl uorescence assay, butwas easier to perform and did not require sophisticated equipment. 2004 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42335202 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.2 Vol.35 |
| format | Artículo científico |
| id | redalyc_42335202 |
| institution | Redalyc |
| language | en |
| publishDate | 2004 |
| publisher | Universidad Nacional Autónoma de México |
| spellingShingle | Identificación de Mycoplasma spp en leche bovina con una prueba de inmunoperoxidasa Fidel Infante Martínez Multidisciplinaria (Ciencias Naturales y Exactas) mycoplasma bovine milk immunoperoxidase assay immunofluorescence assay Identificación de Mycoplasma spp en leche bovina con una prueba de inmunoperoxidasa Fidel Infante Martínez Gerardo H. Flores Gutiérrez Javier Lozoya Chávez Multidisciplinaria (Ciencias Naturales y Exactas) mycoplasma bovine milk immunoperoxidase assay immunofluorescence assay The objective of this study was to implement an immunoperoxidase assay for the identifi cation of Mycoplasmaspp from bovine milk. Eighty samples of naturally infected milk, with different Mycoplasma spp,were analyzed; the immunofl uorescence assay was used as a gold standard. The sensitivity of the immunoperoxidaseassay was six colony-forming units, with no cross-reactions between the antisera, with 100%epidemiological sensitivity and specifi city, and predictive values; there were no statistical differences (P >0.05) between both tests. The reproducibility of the immunoperoxidase assay was high, obtaining identicalresults four times. Furthermore, it was carried out in less time than the immunofl uorescence assay,requiring only a light microscope to read the results and fewer antisera and conjugates. In this study, theimmunoperoxidase assay proved to have similar diagnostic value to the immunofl uorescence assay, butwas easier to perform and did not require sophisticated equipment. 2004 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42335202 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.2 Vol.35 |
| title | Identificación de Mycoplasma spp en leche bovina con una prueba de inmunoperoxidasa |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) mycoplasma bovine milk immunoperoxidase assay immunofluorescence assay |
| url | https://www.redalyc.org/articulo.oa?id=42335202 |