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| Format: | Artículo científico |
| Language: | en |
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Universidad Nacional Autónoma de México
2006
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| Online Access: | https://www.redalyc.org/articulo.oa?id=42337202 |
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Table of Contents:
- Estudio comparativo de las características genotípicas de cepas de Staphylococcus aureus aisladas de casos de mastitis clínica y subclínica en México Amir El-Sayed Joachim Alber Christoph Lämmler Sybille Jäger Wilfried Wolter Hugo Castañeda-Vázquez Multidisciplinaria (Ciencias Naturales y Exactas) MASTITIS STAPHYLOCOCCUS AUREUS GENOTYPIC CHARACTERISTICS Mastitis is one of the major problems challenging the dairy industry worldwide. Among the various organisms causing mastitis,Staphylococcus aureus is considered to be one of the main pathogens causing this disease. In the present study 40 S. aureusisolated from milk samples of clinical and subclinical mastitic cows from different farms in one area in Mexico, were genotypicallycompared. Using 62 different oligonucleotide primers and PCR reactions were carried out to detect genes for a numberof staphylococcal exoproteins, cell surface proteins, and two classes of the accessory regulator gene Agr. The investigated S.aureus were uniformly positive for the gene segment encoding a S. aureus-specifi c part of the 23S rRNA, the genes encodingthermostable nuclease (nuc), clumping factor (clfA), coagulase (coa) and the gene segments encoding the Xr repetitive regionand the IgG binding region of protein A (spa). All tested strains were additionally positive for the hla, fnbA, ebpS and set1 genesand negative for the sbi, sea, seb, sec, sed, see, seg, seh, sej, tst, eta and etb genes. The remaining genes, including hlb, fnbB,cna (Domain A and B), cap5, cap8, agr class I, agr class II and sei were detected in a variable number of isolates. Signifi cant differencesbetween the S. aureus strains isolated from clinical and subclinical cases could be observed in the size of gene clfA, in thedistribution of amplicon sizes of gene coa and the Xr-encoding gene segment of gene spa and in the occurrence of the geneshlb, fnbB, agr class I and agr class II, respectively. 2006 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42337202 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.2 Vol.37