Transmisión de Mycoplasma hyopneumoniae en una granja con separaciones abiertas o sólidas determinada por la prueba de reacción en cadena de la polimerasa
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| Format: | Artículo científico |
| Language: | en |
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Universidad Nacional Autónoma de México
2006
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| _version_ | 1876474912043958272 |
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| author | Montserrat Torremorell |
| author_facet | Montserrat Torremorell |
| contents | Transmisión de Mycoplasma hyopneumoniae en una granja con separaciones abiertas o sólidas determinada por la prueba de reacción en cadena de la polimerasa Montserrat Torremorell Carlos Pijoan Susana Mendoza Álvaro Ruiz Multidisciplinaria (Ciencias Naturales y Exactas) PIG PCR UNIDADES MYCOPLASMA HYOPNEUMONIAE The dynamics of transmission of Mycoplasma hyopneumoniae are described in a conventional nursery fi tted with two differentpen partition designs (open vs. solid), as variables that infl uences the spread of this organism. This study was conducted in a farmknown to be M. hyopneumoniae positive with a 50% prevalence of PCR positive pigs at weaning. This farm had two nursery rooms(6 pens/room): one room was left with the original open pen partitions; pens in the other room were modifi ed by installing solidpartitions using washable plywood. In addition, two groups of pigs (16 pigs/group) were purchased from a M. hyopneumoniaenegative farm (test pigs) and placed in alternate pens in the nursery rooms (2 pens/room). The rest of the pens were fi lled withpigs from the home farm (10 pigs/pen). Test pigs and two homegrown pigs from each pen were sampled throughout the nurseryperiod by nasal swabbing; blood samples were also taken. The nasal swabs were then analyzed by nested-PCR and the bloodsamples by the tween-ELISA. Results revealed no statistic differences in M. hyopneumoniae transmission between groups housedeither in the open or solid pen partitions rooms. Results also showed that M. hyopneumoniae was transmitted slowly within thenursery rooms up to a point where a sudden increase of infection occurred.Transmission of M. hyopneumoniae in confi ned nurseryrooms is not affected by pen partition design. Transmission between infected and control pigs appears to be affected by factorsthat infl uence microbial shedding. PCR proved to be a useful tool to detect the transmission of M. hyopneumoniae in the lactationrooms of the farms. 2006 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42337203 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.2 Vol.37 |
| format | Artículo científico |
| id | redalyc_42337203 |
| institution | Redalyc |
| language | en |
| publishDate | 2006 |
| publisher | Universidad Nacional Autónoma de México |
| spellingShingle | Transmisión de Mycoplasma hyopneumoniae en una granja con separaciones abiertas o sólidas determinada por la prueba de reacción en cadena de la polimerasa Montserrat Torremorell Multidisciplinaria (Ciencias Naturales y Exactas) PIG PCR UNIDADES MYCOPLASMA HYOPNEUMONIAE Transmisión de Mycoplasma hyopneumoniae en una granja con separaciones abiertas o sólidas determinada por la prueba de reacción en cadena de la polimerasa Montserrat Torremorell Carlos Pijoan Susana Mendoza Álvaro Ruiz Multidisciplinaria (Ciencias Naturales y Exactas) PIG PCR UNIDADES MYCOPLASMA HYOPNEUMONIAE The dynamics of transmission of Mycoplasma hyopneumoniae are described in a conventional nursery fi tted with two differentpen partition designs (open vs. solid), as variables that infl uences the spread of this organism. This study was conducted in a farmknown to be M. hyopneumoniae positive with a 50% prevalence of PCR positive pigs at weaning. This farm had two nursery rooms(6 pens/room): one room was left with the original open pen partitions; pens in the other room were modifi ed by installing solidpartitions using washable plywood. In addition, two groups of pigs (16 pigs/group) were purchased from a M. hyopneumoniaenegative farm (test pigs) and placed in alternate pens in the nursery rooms (2 pens/room). The rest of the pens were fi lled withpigs from the home farm (10 pigs/pen). Test pigs and two homegrown pigs from each pen were sampled throughout the nurseryperiod by nasal swabbing; blood samples were also taken. The nasal swabs were then analyzed by nested-PCR and the bloodsamples by the tween-ELISA. Results revealed no statistic differences in M. hyopneumoniae transmission between groups housedeither in the open or solid pen partitions rooms. Results also showed that M. hyopneumoniae was transmitted slowly within thenursery rooms up to a point where a sudden increase of infection occurred.Transmission of M. hyopneumoniae in confi ned nurseryrooms is not affected by pen partition design. Transmission between infected and control pigs appears to be affected by factorsthat infl uence microbial shedding. PCR proved to be a useful tool to detect the transmission of M. hyopneumoniae in the lactationrooms of the farms. 2006 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42337203 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.2 Vol.37 |
| title | Transmisión de Mycoplasma hyopneumoniae en una granja con separaciones abiertas o sólidas determinada por la prueba de reacción en cadena de la polimerasa |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) PIG PCR UNIDADES MYCOPLASMA HYOPNEUMONIAE |
| url | https://www.redalyc.org/articulo.oa?id=42337203 |