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| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad Nacional Autónoma de México
2006
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| Online Access: | https://www.redalyc.org/articulo.oa?id=42337204 |
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Table of Contents:
- Detección de secuencias nucleotídicas de Mycobacterium bovis a partir de adn de moco nasal de caprinos inoculados experimentalmente Isaura Carolina Ramírez Casillas Marco A. Santillán Flores Beatriz Arellano Reynoso Francisco Morales Álvarez Víctor R. Tenorio Gutiérrez Multidisciplinaria (Ciencias Naturales y Exactas) PCR BOVIS NASAL MUCUS BOVINE TUBERCULOSIS Excretion of M. bovis through aerosols and their inhalation is considered the main route of transmission and infection in cattle.The traditional methods used for monitoring the elimination of the microorganism by the nasal route are the bacteriologicalculture and the radiometric method. Recently developed molecular biology methods have favored the fast and reliable diagnosisof mycobacteria in the fi eld. In the present work, the M-PCR technique was used to determine the time at which M. bovis DNAcould be detected from the nasal mucus of 14 experimentally-infected goats. Samples were taken from the nasal cavity at differenttime intervals after the challenge, and bacteriological culture and DNA amplifi cation by M-PCR were carried out. Sixtydays after challenge, the M-PCR techniques detected six out of 14 animals as positive and at day 90, these same six animals plusfour more were detected as positive. No sample was positive to the bacteriological culture. It is known that the bacteriologicalisolation of mycobacteria is diffi cult and that the reduced number of viable bacteria in each sample infl uences the result of thediagnosis. In this work, the advantage of using the M-PCR technique in order to obtain positive results in just a short time wasdemonstrated, which favors a quick and timely identifi cation of the animals that represent a risk of infection in the herd and thatcould represent reservoirs of the disease. 2006 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42337204 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.2 Vol.37