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| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad Nacional Autónoma de México
2006
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| Online Access: | https://www.redalyc.org/articulo.oa?id=42337206 |
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Table of Contents:
- Análisis in vitro de la actividad antibacteriana Oedogonium capillare contra bacterias patógenas de peces Guadalupe Figueroa Pilar Negrete Redondo Jorge Romero Jarero Roxana López Simeón Multidisciplinaria (Ciencias Naturales y Exactas) PLASMIDS AQUACULTURE ANTIBIOTICS NATURAL EXTRACTS OEDOGONIUM CAPILLARE The present study proved in vitro the capability of an extract of Oedogonium capillare, a fresh water green algae, to be an effectiveantibacterial agent against 23 different bacterial species of Enterobacteriaceae, Pseudomonadaceae, Aeromonadaceae andVibrionaceae families, that are pathogens in humans and important in aquaculture. All the different wild strains were isolatedfrom Carassius auratus fi sh cultivated in fi sh farms in the state of Morelos, Mexico. After being purifi ed the strains were identi-fi ed through the API-20E and API-20NE methods. The algae were collected from the ponds located in the Center of Biologicaland Aquaculture Researches in Xochimilco, Mexico. After the algae had been dried and homogenized, they were subjected tohexane extractions at refl ux temperature. The second extraction was made using a chromatographic column of silica gel andethyl-chlorophorm. In order to determine the susceptibility of bacteria to the activity of the extract a standardized diffusiondisk test was carried out. Filtered paper disks were saturated with the extract obtained from the O. capillare algae and commercialantibiotics widely used in the fi sh industry such as: kanamycin, chloramphenicol and tetracycline. In order to determine thepresence or R-plasmid in the strains, the alkaline lysis technique, was used. The whole experiment was mirrored using strains ofthe American Type Culture Collection. The antibacterial behavior of O. capillare was compared with each of the aforementionedcommercial antibiotics with a correlation analysis. High correlation coeffi cients were obtained between the activity of the algaeextract and the antibiotics used in this study. Of the three antibiotics studied, kanamycin was found to be the most related oneto the antibacterial activity of O. capillare. A higher antibacterial activity of the extract was found on the collection strains. Theaverage diameter of the inhibition halos in the cultures of the four bacterial families of this group was greater than that of thewild strains, possibly due to the presence of R-plasmids in the latter group. 2006 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42337206 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.2 Vol.37