Regeneración del nervio ciático axotomizado del perro con prótesis de quitosana precargadas con pregnenolona y la técnica de tubulización
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| Format: | Artículo científico |
| Sprache: | es |
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Universidad Nacional Autónoma de México
2008
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| _version_ | 1876448481594310656 |
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| author | Manuel Rosales Cortés |
| author_facet | Manuel Rosales Cortés |
| contents | Regeneración del nervio ciático axotomizado del perro con prótesis de quitosana precargadas con pregnenolona y la técnica de tubulización Manuel Rosales Cortés Jorge Peregrina Sandoval Jorge Hernández Mercado Guillermo Nolasco Rodríguez María Estela Chávez Delgado Ulises Gómez Pinedo Esther Albarrán Rodríguez Multidisciplinaria (Ciencias Naturales y Exactas) CHITOSAN BIOMATERIAL REGENERATION AXOTOMIZED SCIATIC NERVE PERIPHERAL NERVES AND PREGNENOLONE Peripheral nerves can be injured by traumatisms or mechanical causes, and thermal, ischemic or tumoral damage, this could presentinconveniences of the mobility, sensibility and loss of motor function at the denervated area. The surgical techniques appliedto repair nerves have gone through several phases of evolution such as the tubulization technique which consists of a prosthesiswith a pipe shape at the segment injured. In this work the biomaterial utilized to manufacture the prosthesis was chitosan, sincethis compound allows to incorporate to its matrix promoting nervous growing substances that are released in situ for a longtime while being degraded by titular lysosomes of organic origin. It is known, by diverse studies, that several neurosteroids areinvolved during the regeneration process of peripheral nerves, which functions are already described, as pregnenolone. In orderto determine if there was or not regeneration and the degree of maturity of this ,12 young French Poodle female dogs of 1 to 1.5years old were used (four were used as intact control group, four were subjected to tubulization technique with chitosan, andfour went into tubulization technique with chitosan and pregnenolone neurosteroid, which is a stem hormone), in order to correct15 mm of axotomized segment. The regenerated nerves were evaluated by means of electronic microscopy of transmissionand light, performing cross cuts of 60-70 nm and 1 µm in thickness for their histological analysis. The morphological fi ndingsshowed a similar structure to that of an intact nerve, since the number of myelinated axons, not myelinated and the proportiong (which indicates the ripening degree of the axon), were similar, indicating that the regeneration of the axotomized nerves andtubulization was achieved, independently of the administered treatment 2008 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42339105 es http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.1 Vol.39 |
| format | Artículo científico |
| id | redalyc_42339105 |
| institution | Redalyc |
| language | es |
| publishDate | 2008 |
| publisher | Universidad Nacional Autónoma de México |
| spellingShingle | Regeneración del nervio ciático axotomizado del perro con prótesis de quitosana precargadas con pregnenolona y la técnica de tubulización Manuel Rosales Cortés Multidisciplinaria (Ciencias Naturales y Exactas) CHITOSAN BIOMATERIAL REGENERATION AXOTOMIZED SCIATIC NERVE PERIPHERAL NERVES AND PREGNENOLONE Regeneración del nervio ciático axotomizado del perro con prótesis de quitosana precargadas con pregnenolona y la técnica de tubulización Manuel Rosales Cortés Jorge Peregrina Sandoval Jorge Hernández Mercado Guillermo Nolasco Rodríguez María Estela Chávez Delgado Ulises Gómez Pinedo Esther Albarrán Rodríguez Multidisciplinaria (Ciencias Naturales y Exactas) CHITOSAN BIOMATERIAL REGENERATION AXOTOMIZED SCIATIC NERVE PERIPHERAL NERVES AND PREGNENOLONE Peripheral nerves can be injured by traumatisms or mechanical causes, and thermal, ischemic or tumoral damage, this could presentinconveniences of the mobility, sensibility and loss of motor function at the denervated area. The surgical techniques appliedto repair nerves have gone through several phases of evolution such as the tubulization technique which consists of a prosthesiswith a pipe shape at the segment injured. In this work the biomaterial utilized to manufacture the prosthesis was chitosan, sincethis compound allows to incorporate to its matrix promoting nervous growing substances that are released in situ for a longtime while being degraded by titular lysosomes of organic origin. It is known, by diverse studies, that several neurosteroids areinvolved during the regeneration process of peripheral nerves, which functions are already described, as pregnenolone. In orderto determine if there was or not regeneration and the degree of maturity of this ,12 young French Poodle female dogs of 1 to 1.5years old were used (four were used as intact control group, four were subjected to tubulization technique with chitosan, andfour went into tubulization technique with chitosan and pregnenolone neurosteroid, which is a stem hormone), in order to correct15 mm of axotomized segment. The regenerated nerves were evaluated by means of electronic microscopy of transmissionand light, performing cross cuts of 60-70 nm and 1 µm in thickness for their histological analysis. The morphological fi ndingsshowed a similar structure to that of an intact nerve, since the number of myelinated axons, not myelinated and the proportiong (which indicates the ripening degree of the axon), were similar, indicating that the regeneration of the axotomized nerves andtubulization was achieved, independently of the administered treatment 2008 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42339105 es http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.1 Vol.39 |
| title | Regeneración del nervio ciático axotomizado del perro con prótesis de quitosana precargadas con pregnenolona y la técnica de tubulización |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) CHITOSAN BIOMATERIAL REGENERATION AXOTOMIZED SCIATIC NERVE PERIPHERAL NERVES AND PREGNENOLONE |
| url | https://www.redalyc.org/articulo.oa?id=42339105 |