Saved in:
| Main Author: | |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad Nacional Autónoma de México
2008
|
| Subjects: | |
| Online Access: | https://www.redalyc.org/articulo.oa?id=42339308 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1866584982749708288 |
|---|---|
| author | Francisco J. Díaz García |
| author_facet | Francisco J. Díaz García |
| contents | Secuenciación de un fragmento de ADNc homólogo a interleucina-1 alfa humana derivado de leucocitos de armadillo (Dasypus novemcinctus) Francisco J. Díaz García Saúl Flores Medina Fernando M. Guerra Infante Multidisciplinaria (Ciencias Naturales y Exactas) RT PCR SEQUENCING INTERLEUKIN DASYPUS NOVEMCINCTUS Messenger RNA (RNAm) detection was done by a reverse-transcription assay coupled to the polymerase chain reaction (RT-PCR)specifi c for human interleukin-1 alpha on armadillo leukocytes stimulated with phorbol myristate acetate (PMA). This strategyallowed amplifying a DNA fragment of 491 bp. Furthermore, the sequence showed high nucleotide homology with the human(99%), monkey (93%), pig (82%), horse (81%) and llama (81%) cDNA. Meanwhile, the deduced amino acid sequence was compatiblewith the precursor of the human interleukin-1 alpha. These results suggest the presence of the interleukin-1 gene in thearmadillo genome. However, it is necessary to characterize the complete sequence of the gene and prove the functionality of thetranslated protein to clarify the immune response mechanisms in the armadillo against Mycobacterium leprae 2008 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42339308 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.3 Vol.39 |
| format | Artículo científico |
| id | redalyc_42339308 |
| language | en |
| publishDate | 2008 |
| publisher | Universidad Nacional Autónoma de México |
| spellingShingle | Secuenciación de un fragmento de ADNc homólogo a interleucina-1 alfa humana derivado de leucocitos de armadillo (Dasypus novemcinctus) Francisco J. Díaz García Multidisciplinaria (Ciencias Naturales y Exactas) RT PCR SEQUENCING INTERLEUKIN DASYPUS NOVEMCINCTUS Secuenciación de un fragmento de ADNc homólogo a interleucina-1 alfa humana derivado de leucocitos de armadillo (Dasypus novemcinctus) Francisco J. Díaz García Saúl Flores Medina Fernando M. Guerra Infante Multidisciplinaria (Ciencias Naturales y Exactas) RT PCR SEQUENCING INTERLEUKIN DASYPUS NOVEMCINCTUS Messenger RNA (RNAm) detection was done by a reverse-transcription assay coupled to the polymerase chain reaction (RT-PCR)specifi c for human interleukin-1 alpha on armadillo leukocytes stimulated with phorbol myristate acetate (PMA). This strategyallowed amplifying a DNA fragment of 491 bp. Furthermore, the sequence showed high nucleotide homology with the human(99%), monkey (93%), pig (82%), horse (81%) and llama (81%) cDNA. Meanwhile, the deduced amino acid sequence was compatiblewith the precursor of the human interleukin-1 alpha. These results suggest the presence of the interleukin-1 gene in thearmadillo genome. However, it is necessary to characterize the complete sequence of the gene and prove the functionality of thetranslated protein to clarify the immune response mechanisms in the armadillo against Mycobacterium leprae 2008 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42339308 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.3 Vol.39 |
| title | Secuenciación de un fragmento de ADNc homólogo a interleucina-1 alfa humana derivado de leucocitos de armadillo (Dasypus novemcinctus) |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) RT PCR SEQUENCING INTERLEUKIN DASYPUS NOVEMCINCTUS |
| url | https://www.redalyc.org/articulo.oa?id=42339308 |