Molecular detection of Anaplasma marginale in stable flies Stomoxys calcitrans (Diptera: Muscidae) feeding on a tick-free bovine herd
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| Format: | Artículo científico |
| Langue: | en |
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Universidad Nacional Autónoma de México
2018
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| _version_ | 1876470893811597312 |
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| author | Carlos R. Bautista |
| author_facet | Carlos R. Bautista |
| contents | Molecular detection of Anaplasma marginale in stable flies Stomoxys calcitrans (Diptera: Muscidae) feeding on a tick-free bovine herd Carlos R. Bautista Tania Rodríguez Carmen Rojas José J. Lira Jesús A. Álvarez Diego Polanco Multidisciplinaria (Ciencias Naturales y Exactas) nested PCR Anaplasma marginale Stomoxys calcitrans Data on the implication of the stable fly (Stomoxys calcitrans, Diptera: Muscidae), in the transmission of Anaplasma marginale in tick-free cattle is scarce. Hence, the objective of this investigation was to detect the presence of DNA from A. marginale in stable flies caught near a bovine herd, which has been maintained free of ticks for 40 years, and occasionally presents some clinical cases of anaplasmosis. Twenty-four batches of S. calcitrans (15 flies each) were collected in the morning and in the afternoon twice a week (except for one catch in one week of September and one catch in one week of December) during 12 catching days in a period of four months. Their DNA was obtained and analysed by nested PCR (nPCR) to identify the presence of A. marginale DNA. Seven of the batches (29.16 %) were positive for A. marginale, as detected by an nPCR that targets the A. marginale msp5 gene. Similarly, in two out of 12 catching days (16.66 %), those S. calcitrans batches collected in the morning and in the afternoon, were positive for A. marginale, while those S. calcitrans groups collected in three out of 12 catching days (25 %), were positive for the rickettsia. The obtained results suggest that A. marginale transmission is carried out mechanically by S. calcitrans, favouring the circulation and maintenance of the microorganism in this particular bovine herd. 2018 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42371695001 https://www.redalyc.org/journal/423/42371695001/ https://www.redalyc.org/journal/423/42371695001/html/ https://www.redalyc.org/journal/423/42371695001/42371695001.epub https://www.redalyc.org/journal/423/42371695001/movil 10.21753/vmoa.5.1.436 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.1 Vol.5 |
| format | Artículo científico |
| id | redalyc_42371695001 |
| institution | Redalyc |
| language | en |
| publishDate | 2018 |
| publisher | Universidad Nacional Autónoma de México |
| spellingShingle | Molecular detection of Anaplasma marginale in stable flies Stomoxys calcitrans (Diptera: Muscidae) feeding on a tick-free bovine herd Carlos R. Bautista Multidisciplinaria (Ciencias Naturales y Exactas) nested PCR Anaplasma marginale Stomoxys calcitrans Molecular detection of Anaplasma marginale in stable flies Stomoxys calcitrans (Diptera: Muscidae) feeding on a tick-free bovine herd Carlos R. Bautista Tania Rodríguez Carmen Rojas José J. Lira Jesús A. Álvarez Diego Polanco Multidisciplinaria (Ciencias Naturales y Exactas) nested PCR Anaplasma marginale Stomoxys calcitrans Data on the implication of the stable fly (Stomoxys calcitrans, Diptera: Muscidae), in the transmission of Anaplasma marginale in tick-free cattle is scarce. Hence, the objective of this investigation was to detect the presence of DNA from A. marginale in stable flies caught near a bovine herd, which has been maintained free of ticks for 40 years, and occasionally presents some clinical cases of anaplasmosis. Twenty-four batches of S. calcitrans (15 flies each) were collected in the morning and in the afternoon twice a week (except for one catch in one week of September and one catch in one week of December) during 12 catching days in a period of four months. Their DNA was obtained and analysed by nested PCR (nPCR) to identify the presence of A. marginale DNA. Seven of the batches (29.16 %) were positive for A. marginale, as detected by an nPCR that targets the A. marginale msp5 gene. Similarly, in two out of 12 catching days (16.66 %), those S. calcitrans batches collected in the morning and in the afternoon, were positive for A. marginale, while those S. calcitrans groups collected in three out of 12 catching days (25 %), were positive for the rickettsia. The obtained results suggest that A. marginale transmission is carried out mechanically by S. calcitrans, favouring the circulation and maintenance of the microorganism in this particular bovine herd. 2018 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42371695001 https://www.redalyc.org/journal/423/42371695001/ https://www.redalyc.org/journal/423/42371695001/html/ https://www.redalyc.org/journal/423/42371695001/42371695001.epub https://www.redalyc.org/journal/423/42371695001/movil 10.21753/vmoa.5.1.436 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.1 Vol.5 |
| title | Molecular detection of Anaplasma marginale in stable flies Stomoxys calcitrans (Diptera: Muscidae) feeding on a tick-free bovine herd |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) nested PCR Anaplasma marginale Stomoxys calcitrans |
| url | https://www.redalyc.org/articulo.oa?id=42371695001 https://www.redalyc.org/journal/423/42371695001/ https://www.redalyc.org/journal/423/42371695001/html/ https://www.redalyc.org/journal/423/42371695001/42371695001.epub https://www.redalyc.org/journal/423/42371695001/movil |