Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos
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| Format: | Artículo científico |
| Language: | en |
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Universidad Nacional Autónoma de México
2018
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| _version_ | 1876443947539103744 |
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| author | José Luis Martínez-Ibarra |
| author_facet | José Luis Martínez-Ibarra |
| contents | Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos José Luis Martínez-Ibarra Eugenia Adriana Espinoza-Mendoza Raymundo Rangel-Santos Demetrio Alonso Ambriz-Garcia María del Carmen Navarro-Maldonado Multidisciplinaria (Ciencias Naturales y Exactas) Ovis aries Resveratrol Handmade cloning Embryo development In vitro maturation The effect of resveratrol on the in vitro maturation (IVM) of ovine (Ovis aries) oocytes and the development of handmade cloned embryos was evaluated. The nuclear maturation and reactive oxygen species (ROS) levels in the oocytes, as well as the early development and morphological cloned embryo quality, were evaluated under different resveratrol concentrations (0, 0.5, 2 and 5 µM). After IVM, no significant difference was observed in the maturation rate of oocytes treated with 0.5 µM (81.3 %) and 2 µM (72 %) resveratrol compared to that of the control group (0 µM) (74.2 %), but the rate significantly decreased at 5 µM (56 %) (p < 0.05). When the oocyte ROS levels were determined, no significant differences among the groups were observed (p > 0.05). For cloned embryo development, the embryos obtained from the oocytes treated with 0.5 µM resveratrol showed higher (p < 0.05) compacted morula rates (10.7 %) compared to the embryos obtained from the oocytes treated with 0, 2 and 5 µM (6.2, 0 and 0 %, respectively). Regarding embryo morphological quality, the embryos from the oocytes treated with 0.5 µM resveratrol showed a lower rate of poor quality morulae (4.7 %) in comparison to those treated with 0, 2 and 5 µM (23.8, 23.3 and 33.3 %, respectively) (p < 0.05). In conclusion, resveratrol showed no significant improvement on the IVM or ROS levels in domestic ovine oocytes. However, treatment with 0.5 µM resveratrol during IVM improved embryo quality and promoted morulae compaction of Ovis aries handmade cloned embryos. 2018 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42371698002 https://www.redalyc.org/journal/423/42371698002/ https://www.redalyc.org/journal/423/42371698002/html/ https://www.redalyc.org/journal/423/42371698002/42371698002.epub https://www.redalyc.org/journal/423/42371698002/movil 10.22201/fmvz.24486760e.2018.4.491 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.4 Vol.5 |
| format | Artículo científico |
| id | redalyc_42371698002 |
| institution | Redalyc |
| language | en |
| publishDate | 2018 |
| publisher | Universidad Nacional Autónoma de México |
| spellingShingle | Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos José Luis Martínez-Ibarra Multidisciplinaria (Ciencias Naturales y Exactas) Ovis aries Resveratrol Handmade cloning Embryo development In vitro maturation Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos José Luis Martínez-Ibarra Eugenia Adriana Espinoza-Mendoza Raymundo Rangel-Santos Demetrio Alonso Ambriz-Garcia María del Carmen Navarro-Maldonado Multidisciplinaria (Ciencias Naturales y Exactas) Ovis aries Resveratrol Handmade cloning Embryo development In vitro maturation The effect of resveratrol on the in vitro maturation (IVM) of ovine (Ovis aries) oocytes and the development of handmade cloned embryos was evaluated. The nuclear maturation and reactive oxygen species (ROS) levels in the oocytes, as well as the early development and morphological cloned embryo quality, were evaluated under different resveratrol concentrations (0, 0.5, 2 and 5 µM). After IVM, no significant difference was observed in the maturation rate of oocytes treated with 0.5 µM (81.3 %) and 2 µM (72 %) resveratrol compared to that of the control group (0 µM) (74.2 %), but the rate significantly decreased at 5 µM (56 %) (p < 0.05). When the oocyte ROS levels were determined, no significant differences among the groups were observed (p > 0.05). For cloned embryo development, the embryos obtained from the oocytes treated with 0.5 µM resveratrol showed higher (p < 0.05) compacted morula rates (10.7 %) compared to the embryos obtained from the oocytes treated with 0, 2 and 5 µM (6.2, 0 and 0 %, respectively). Regarding embryo morphological quality, the embryos from the oocytes treated with 0.5 µM resveratrol showed a lower rate of poor quality morulae (4.7 %) in comparison to those treated with 0, 2 and 5 µM (23.8, 23.3 and 33.3 %, respectively) (p < 0.05). In conclusion, resveratrol showed no significant improvement on the IVM or ROS levels in domestic ovine oocytes. However, treatment with 0.5 µM resveratrol during IVM improved embryo quality and promoted morulae compaction of Ovis aries handmade cloned embryos. 2018 artículo científico 0301-5092 https://www.redalyc.org/articulo.oa?id=42371698002 https://www.redalyc.org/journal/423/42371698002/ https://www.redalyc.org/journal/423/42371698002/html/ https://www.redalyc.org/journal/423/42371698002/42371698002.epub https://www.redalyc.org/journal/423/42371698002/movil 10.22201/fmvz.24486760e.2018.4.491 en http://www.redalyc.org/revista.oa?id=423 Veterinaria México application/pdf Universidad Nacional Autónoma de México Veterinaria México (México) Num.4 Vol.5 |
| title | Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) Ovis aries Resveratrol Handmade cloning Embryo development In vitro maturation |
| url | https://www.redalyc.org/articulo.oa?id=42371698002 https://www.redalyc.org/journal/423/42371698002/ https://www.redalyc.org/journal/423/42371698002/html/ https://www.redalyc.org/journal/423/42371698002/42371698002.epub https://www.redalyc.org/journal/423/42371698002/movil |