Amperometric biosensor for ascorbic acid
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Universidade Estadual Paulista Júlio de Mesquita Filho
2005
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| _version_ | 1876435694047461376 |
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| author | I. N. Tomita |
| author_facet | I. N. Tomita |
| contents | Amperometric biosensor for ascorbic acid I. N. Tomita A. Manzoli F. L. Fertonani H. Yamanaka Química biosensor ascorbic acid flow injection analysis A L-ascorbic acid biosensor based on ascorbate oxidase has been developed. The enzyme wasextracted from the mesocarp of cucumber (Cucumis sativus) by using 0.05 mol L-1 phosphate buffer, pH5.8 containing 0.5 mol L-1 NaCl. After the dialysis versus phosphate buffer0.05 mol L-1 pH 5.8, the enzyme was immobilized onto nylon net through glutaraldehyde covalentbond. The membrane was coupled to an O2 electrode and the yielding reaction monitored by oxygendepletion at -600 mV using flow injection analysis optimized to 0.1 mol L-1 phosphate buffer pH 5.8, as thecarrier solution and flow-rate of 0.5 mL min-1. The ascorbic acid calibration curve was linear from 1.2x10-4 to1.0x10-3 mol L-1. The evaluation of biosensor lifetime leads to 500 injections. Commercial pharmaceuticalsamples were analyzed with the proposed method and the results were compared with those obtained byhigh-performance liquid chromatography (HPLC). 2005 artículo científico 0100-4670 https://www.redalyc.org/articulo.oa?id=42930205 en http://www.redalyc.org/revista.oa?id=429 Eclética Química application/pdf Universidade Estadual Paulista Júlio de Mesquita Filho Eclética Química (Brasil) Num.2 Vol.30 |
| format | Artículo científico |
| id | redalyc_42930205 |
| institution | Redalyc |
| language | en |
| publishDate | 2005 |
| publisher | Universidade Estadual Paulista Júlio de Mesquita Filho |
| spellingShingle | Amperometric biosensor for ascorbic acid I. N. Tomita Química biosensor ascorbic acid flow injection analysis Amperometric biosensor for ascorbic acid I. N. Tomita A. Manzoli F. L. Fertonani H. Yamanaka Química biosensor ascorbic acid flow injection analysis A L-ascorbic acid biosensor based on ascorbate oxidase has been developed. The enzyme wasextracted from the mesocarp of cucumber (Cucumis sativus) by using 0.05 mol L-1 phosphate buffer, pH5.8 containing 0.5 mol L-1 NaCl. After the dialysis versus phosphate buffer0.05 mol L-1 pH 5.8, the enzyme was immobilized onto nylon net through glutaraldehyde covalentbond. The membrane was coupled to an O2 electrode and the yielding reaction monitored by oxygendepletion at -600 mV using flow injection analysis optimized to 0.1 mol L-1 phosphate buffer pH 5.8, as thecarrier solution and flow-rate of 0.5 mL min-1. The ascorbic acid calibration curve was linear from 1.2x10-4 to1.0x10-3 mol L-1. The evaluation of biosensor lifetime leads to 500 injections. Commercial pharmaceuticalsamples were analyzed with the proposed method and the results were compared with those obtained byhigh-performance liquid chromatography (HPLC). 2005 artículo científico 0100-4670 https://www.redalyc.org/articulo.oa?id=42930205 en http://www.redalyc.org/revista.oa?id=429 Eclética Química application/pdf Universidade Estadual Paulista Júlio de Mesquita Filho Eclética Química (Brasil) Num.2 Vol.30 |
| title | Amperometric biosensor for ascorbic acid |
| topic | Química biosensor ascorbic acid flow injection analysis |
| url | https://www.redalyc.org/articulo.oa?id=42930205 |