Chiral separations of mandelic acid by HPLC using molecularly imprinted polymers
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| Format: | Artículo científico |
| Language: | en |
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Universidade Estadual Paulista Júlio de Mesquita Filho
2005
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| _version_ | 1876443665424973824 |
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| author | Chin-Yin Hung |
| author_facet | Chin-Yin Hung |
| contents | Chiral separations of mandelic acid by HPLC using molecularly imprinted polymers Chin-Yin Hung Han-Hung Huang Ching-Chiang Hwang Química (±) enantiomers mandelic acid chromatographic resolution molecular imprinting technique Styrene is used in a variety of chemical industries. Environmental and occupational exposuresto styrene occur predominantly through inhalation. The major metabolite of styrene is present in twoenantiomeric forms, chiral R- and S- hydroxy-1-phenyl-acetic acid (R-and S-mandelic acid, MA). Thus, theconcentration of MA, particularly of its enantiomers, has been used in urine tests to determine whetherworkers have been exposed to styrene.This study describes a method of analyzing mandelic acid using molecular imprinting techniques andHPLC detection to perform the separation of diastereoisomers of mandelic acid. The molecularly imprintedpolymer (MIP) was prepared by non-covalent molecular imprinting using (+) MA, (-) MA or (+)phenylalanine, (-) phenylalanine as templates. Methacrylic acid (MAA) and ethylene glycol dimethacrylate(EGDMA) were copolymerized in the presence of the template molecules. The bulk polymerization wascarried out at 4oC under UV radiation. The resulting MIP was grounded into 25~44¼m particles, whichwere slurry packed into analytical columns. After the template molecules were removed, the MIP-packedcolumns were found to be effective for the chromatographic resolution of (±)-mandelic acid. This methodis simpler and more convenient than other chromatographic methods. 2005 artículo científico 0100-4670 https://www.redalyc.org/articulo.oa?id=42930409 en http://www.redalyc.org/revista.oa?id=429 Eclética Química application/pdf Universidade Estadual Paulista Júlio de Mesquita Filho Eclética Química (Brasil) Num.4 Vol.30 |
| format | Artículo científico |
| id | redalyc_42930409 |
| institution | Redalyc |
| language | en |
| publishDate | 2005 |
| publisher | Universidade Estadual Paulista Júlio de Mesquita Filho |
| spellingShingle | Chiral separations of mandelic acid by HPLC using molecularly imprinted polymers Chin-Yin Hung Química (±) enantiomers mandelic acid chromatographic resolution molecular imprinting technique Chiral separations of mandelic acid by HPLC using molecularly imprinted polymers Chin-Yin Hung Han-Hung Huang Ching-Chiang Hwang Química (±) enantiomers mandelic acid chromatographic resolution molecular imprinting technique Styrene is used in a variety of chemical industries. Environmental and occupational exposuresto styrene occur predominantly through inhalation. The major metabolite of styrene is present in twoenantiomeric forms, chiral R- and S- hydroxy-1-phenyl-acetic acid (R-and S-mandelic acid, MA). Thus, theconcentration of MA, particularly of its enantiomers, has been used in urine tests to determine whetherworkers have been exposed to styrene.This study describes a method of analyzing mandelic acid using molecular imprinting techniques andHPLC detection to perform the separation of diastereoisomers of mandelic acid. The molecularly imprintedpolymer (MIP) was prepared by non-covalent molecular imprinting using (+) MA, (-) MA or (+)phenylalanine, (-) phenylalanine as templates. Methacrylic acid (MAA) and ethylene glycol dimethacrylate(EGDMA) were copolymerized in the presence of the template molecules. The bulk polymerization wascarried out at 4oC under UV radiation. The resulting MIP was grounded into 25~44¼m particles, whichwere slurry packed into analytical columns. After the template molecules were removed, the MIP-packedcolumns were found to be effective for the chromatographic resolution of (±)-mandelic acid. This methodis simpler and more convenient than other chromatographic methods. 2005 artículo científico 0100-4670 https://www.redalyc.org/articulo.oa?id=42930409 en http://www.redalyc.org/revista.oa?id=429 Eclética Química application/pdf Universidade Estadual Paulista Júlio de Mesquita Filho Eclética Química (Brasil) Num.4 Vol.30 |
| title | Chiral separations of mandelic acid by HPLC using molecularly imprinted polymers |
| topic | Química (±) enantiomers mandelic acid chromatographic resolution molecular imprinting technique |
| url | https://www.redalyc.org/articulo.oa?id=42930409 |