Spectrophotometric determination of lansoprazole in pharmaceuticals using bromate-bromide mixture based on redox and complexation reactions
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| Format: | Artículo científico |
| Language: | en |
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Universidade Estadual Paulista Júlio de Mesquita Filho
2007
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| author | B.C. Somashekar |
| author_facet | B.C. Somashekar |
| contents | Spectrophotometric determination of lansoprazole in pharmaceuticals using bromate-bromide mixture based on redox and complexation reactions B.C. Somashekar U.R. Anil kumar K. Basavaiah V. Ramakrishna Química bromate capsules Lansoprazole determination bromide mixture Two sensitive spectrophotometric methods are described for the determination of lansoprazole (LPZ) in bulk drug and in capsule formulation. The methods are based on the oxidation of lansoprazole by insitu generated bromine followed by determination of unreacted bromine by two different reaction schemes. In one procedure (method A), the residual bromine is treated with excess of iron (II), and the resulting iron (III) is complexed with thiocyanate and measured at 470 nm. The second approach (method B) involves treating the unreacted bromine with a measured excess of iron (II) and remaining iron (II) is complexed with orthophenanthroline at a raised pH, and measured at 510 nm. Inboth methods, the amount of bromine reacted corresponds to the amount of LPZ. The experimental conditions were optimized. In method A, the absorbance is found to decrease linearly with theconcentration of LPZ (r = -0.9986) where as in the method B a linear increase in absorbance occurs (r= 0.9986) The systems obey Beer is law for 0.5-4.0 and 0.5-6.0 µg mLֿ¹ for method A and method B, respectively. The calculated molar absorptivity values are 3.97µ104 and 3.07µ104 L molֿ¹cmֿ¹ for method A and method B, respectively, and the corresponding Sandell sensitivity values are 0.0039 and 0.0013 µg cmֿ². The limit of detection (LOD) and quantification (LOQ) are also reported for bothmethods. Intra-day and inter-day precision, and accuracy of the methods were established as per thecurrent ICH guidelines. The methods were successfully applied to the determination of LPZ in capsulesand the results tallied well with the label claim and the results were statistically compared with those ofa reference method by applying the Student is t-test and F-test. No interference was observed from theconcomitant substances normally added to capsules. The accuracy and validity of the methods were further ascertained by performing recovery experiments via standard-addition method. 2007 artículo científico 0100-4670 https://www.redalyc.org/articulo.oa?id=42932108 en http://www.redalyc.org/revista.oa?id=429 Eclética Química application/pdf Universidade Estadual Paulista Júlio de Mesquita Filho Eclética Química (Brasil) Num.1 Vol.32 |
| format | Artículo científico |
| id | redalyc_42932108 |
| institution | Redalyc |
| language | en |
| publishDate | 2007 |
| publisher | Universidade Estadual Paulista Júlio de Mesquita Filho |
| spellingShingle | Spectrophotometric determination of lansoprazole in pharmaceuticals using bromate-bromide mixture based on redox and complexation reactions B.C. Somashekar Química bromate capsules Lansoprazole determination bromide mixture Spectrophotometric determination of lansoprazole in pharmaceuticals using bromate-bromide mixture based on redox and complexation reactions B.C. Somashekar U.R. Anil kumar K. Basavaiah V. Ramakrishna Química bromate capsules Lansoprazole determination bromide mixture Two sensitive spectrophotometric methods are described for the determination of lansoprazole (LPZ) in bulk drug and in capsule formulation. The methods are based on the oxidation of lansoprazole by insitu generated bromine followed by determination of unreacted bromine by two different reaction schemes. In one procedure (method A), the residual bromine is treated with excess of iron (II), and the resulting iron (III) is complexed with thiocyanate and measured at 470 nm. The second approach (method B) involves treating the unreacted bromine with a measured excess of iron (II) and remaining iron (II) is complexed with orthophenanthroline at a raised pH, and measured at 510 nm. Inboth methods, the amount of bromine reacted corresponds to the amount of LPZ. The experimental conditions were optimized. In method A, the absorbance is found to decrease linearly with theconcentration of LPZ (r = -0.9986) where as in the method B a linear increase in absorbance occurs (r= 0.9986) The systems obey Beer is law for 0.5-4.0 and 0.5-6.0 µg mLֿ¹ for method A and method B, respectively. The calculated molar absorptivity values are 3.97µ104 and 3.07µ104 L molֿ¹cmֿ¹ for method A and method B, respectively, and the corresponding Sandell sensitivity values are 0.0039 and 0.0013 µg cmֿ². The limit of detection (LOD) and quantification (LOQ) are also reported for bothmethods. Intra-day and inter-day precision, and accuracy of the methods were established as per thecurrent ICH guidelines. The methods were successfully applied to the determination of LPZ in capsulesand the results tallied well with the label claim and the results were statistically compared with those ofa reference method by applying the Student is t-test and F-test. No interference was observed from theconcomitant substances normally added to capsules. The accuracy and validity of the methods were further ascertained by performing recovery experiments via standard-addition method. 2007 artículo científico 0100-4670 https://www.redalyc.org/articulo.oa?id=42932108 en http://www.redalyc.org/revista.oa?id=429 Eclética Química application/pdf Universidade Estadual Paulista Júlio de Mesquita Filho Eclética Química (Brasil) Num.1 Vol.32 |
| title | Spectrophotometric determination of lansoprazole in pharmaceuticals using bromate-bromide mixture based on redox and complexation reactions |
| topic | Química bromate capsules Lansoprazole determination bromide mixture |
| url | https://www.redalyc.org/articulo.oa?id=42932108 |