Zinc fractionation in cow, goat, sheep and soybean milk samples using gel-electrophoresis and determination by electrothermal atomic absorption spectrometry (ETAAS)
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidade Estadual Paulista Júlio de Mesquita Filho
2021
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| _version_ | 1876449802525343744 |
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| author | Carla Maíra Bossu |
| author_facet | Carla Maíra Bossu |
| contents | Zinc fractionation in cow, goat, sheep and soybean milk samples using gel-electrophoresis and determination by electrothermal atomic absorption spectrometry (ETAAS) Carla Maíra Bossu Rodrigo Giglioti Marcia Cristina de Sena Oliveira Pedro Vitoriano de Oliveira Ana Rita de Araujo Nogueira Química zinc proteins Fractionation fractionation A screening method for zinc levels in different milk samples (raw cow, raw sheep, UHT cow, UHT goat and soybean milk base) was performed to establish the Zn levels’ differences in protein samples. The samples were digested in a cavity microwave oven and the total Zn levels in the extracts were determined by flame absorption atomic spectrometry (FAAS). The protein separation was performed by urea polyacrylamide gel electrophoresis (UREA-PAGE). Protein bands were digested in the cavity microwave oven and Zn-protein analysis was further conducted by electrothermal atomic absorption spectrometry (ETAAS). The results showed that Zn is mainly bound to 32 kDa (β-casein) protein (17.0 ± 2.0%) in UHT cow and 24 kDa (α-casein) protein (9.0 ± 0.6%) in raw sheep milk. This method provided quantitative information regarding Zn species present in the protein fractions of the milk samples. The accuracy was evaluated using certified reference material (whole milk powder, NIST 8435) with statistically equivalent concentrations (Student’s .-test) for total Zn and by addition and recovery experiments applied to measure Zn-protein. The recovered values were in the 92-110% range. 2021 artículo científico 0100-4670 https://www.redalyc.org/articulo.oa?id=42965151002 https://www.redalyc.org/journal/429/42965151002/ https://www.redalyc.org/journal/429/42965151002/html/ https://www.redalyc.org/journal/429/42965151002/42965151002.epub https://www.redalyc.org/journal/429/42965151002/movil https://doi.org/10.26850/1678-4618eqj.v46.1.2021.p12-20 en http://www.redalyc.org/revista.oa?id=429 Eclética Química application/pdf Universidade Estadual Paulista Júlio de Mesquita Filho Eclética Química (Brasil) Num.1 Vol.46 |
| format | Artículo científico |
| id | redalyc_42965151002 |
| institution | Redalyc |
| language | en |
| publishDate | 2021 |
| publisher | Universidade Estadual Paulista Júlio de Mesquita Filho |
| spellingShingle | Zinc fractionation in cow, goat, sheep and soybean milk samples using gel-electrophoresis and determination by electrothermal atomic absorption spectrometry (ETAAS) Carla Maíra Bossu Química zinc proteins Fractionation fractionation Zinc fractionation in cow, goat, sheep and soybean milk samples using gel-electrophoresis and determination by electrothermal atomic absorption spectrometry (ETAAS) Carla Maíra Bossu Rodrigo Giglioti Marcia Cristina de Sena Oliveira Pedro Vitoriano de Oliveira Ana Rita de Araujo Nogueira Química zinc proteins Fractionation fractionation A screening method for zinc levels in different milk samples (raw cow, raw sheep, UHT cow, UHT goat and soybean milk base) was performed to establish the Zn levels’ differences in protein samples. The samples were digested in a cavity microwave oven and the total Zn levels in the extracts were determined by flame absorption atomic spectrometry (FAAS). The protein separation was performed by urea polyacrylamide gel electrophoresis (UREA-PAGE). Protein bands were digested in the cavity microwave oven and Zn-protein analysis was further conducted by electrothermal atomic absorption spectrometry (ETAAS). The results showed that Zn is mainly bound to 32 kDa (β-casein) protein (17.0 ± 2.0%) in UHT cow and 24 kDa (α-casein) protein (9.0 ± 0.6%) in raw sheep milk. This method provided quantitative information regarding Zn species present in the protein fractions of the milk samples. The accuracy was evaluated using certified reference material (whole milk powder, NIST 8435) with statistically equivalent concentrations (Student’s .-test) for total Zn and by addition and recovery experiments applied to measure Zn-protein. The recovered values were in the 92-110% range. 2021 artículo científico 0100-4670 https://www.redalyc.org/articulo.oa?id=42965151002 https://www.redalyc.org/journal/429/42965151002/ https://www.redalyc.org/journal/429/42965151002/html/ https://www.redalyc.org/journal/429/42965151002/42965151002.epub https://www.redalyc.org/journal/429/42965151002/movil https://doi.org/10.26850/1678-4618eqj.v46.1.2021.p12-20 en http://www.redalyc.org/revista.oa?id=429 Eclética Química application/pdf Universidade Estadual Paulista Júlio de Mesquita Filho Eclética Química (Brasil) Num.1 Vol.46 |
| title | Zinc fractionation in cow, goat, sheep and soybean milk samples using gel-electrophoresis and determination by electrothermal atomic absorption spectrometry (ETAAS) |
| topic | Química zinc proteins Fractionation fractionation |
| url | https://www.redalyc.org/articulo.oa?id=42965151002 https://www.redalyc.org/journal/429/42965151002/ https://www.redalyc.org/journal/429/42965151002/html/ https://www.redalyc.org/journal/429/42965151002/42965151002.epub https://www.redalyc.org/journal/429/42965151002/movil https://doi.org/10.26850/1678-4618eqj.v46.1.2021.p12-20 |