Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Universidad de Antioquia
2020
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| _version_ | 1876435558572490752 |
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| author | Estefanía Echeverri Correa |
| author_facet | Estefanía Echeverri Correa |
| contents | Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution Estefanía Echeverri Correa David Orlando Grajales Lopera Santiago Gutiérrez Restrepo Claudia Patricia Ossa-Orozco Ingeniería dialysis membrane Silk fibroin degumming process tissue engineering Silk from Bombyx mori has two main proteins: fibroin and sericin. Fibroin is a protein that exhibits good biocompatibility and high surface reactivity, desirable properties for many biomedical applications. Sericin is related with adverse immune response in some medical uses. Therefore, its removal (degumming) is desirable in almost all fields, including the textile industry and biological applications. In this study, three degumming methods (distilled water, liquid neutral detergent and sodium carbonate solution) were evaluated using Raman spectroscopy. Degummed silk treated with sodium carbonate solution exhibits a pattern consistent with previous studies for sericin-free fibroin, and significant differences with the untreated silk pattern. Also, degummed fibroin fibers were dissolved in a calcium chloride solution, in pursuit of a more versatile material. Additionally, different porous membranes of dialysis tubes were tested to remove residual salts, and were compared by conductivity measurements and EDS analysis, identifying good performance for an affordable food cellulose membrane. The results showed that it is possible to obtain fibroin with adequate chemical properties, using low-cost process and membrane of dialysis tubes. 2020 artículo científico 0120-6230 https://www.redalyc.org/articulo.oa?id=43062873010 https://www.redalyc.org/journal/430/43062873010/ https://www.redalyc.org/journal/430/43062873010/html/ https://www.redalyc.org/journal/430/43062873010/43062873010.epub https://www.redalyc.org/journal/430/43062873010/movil 10.17533/udea.redin.20190731 en http://www.redalyc.org/revista.oa?id=430 Revista Facultad de Ingeniería Universidad de Antioquia application/pdf Universidad de Antioquia Revista Facultad de Ingeniería Universidad de Antioquia (Colombia) Num.94 |
| format | Artículo científico |
| id | redalyc_43062873010 |
| institution | Redalyc |
| language | en |
| publishDate | 2020 |
| publisher | Universidad de Antioquia |
| spellingShingle | Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution Estefanía Echeverri Correa Ingeniería dialysis membrane Silk fibroin degumming process tissue engineering Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution Estefanía Echeverri Correa David Orlando Grajales Lopera Santiago Gutiérrez Restrepo Claudia Patricia Ossa-Orozco Ingeniería dialysis membrane Silk fibroin degumming process tissue engineering Silk from Bombyx mori has two main proteins: fibroin and sericin. Fibroin is a protein that exhibits good biocompatibility and high surface reactivity, desirable properties for many biomedical applications. Sericin is related with adverse immune response in some medical uses. Therefore, its removal (degumming) is desirable in almost all fields, including the textile industry and biological applications. In this study, three degumming methods (distilled water, liquid neutral detergent and sodium carbonate solution) were evaluated using Raman spectroscopy. Degummed silk treated with sodium carbonate solution exhibits a pattern consistent with previous studies for sericin-free fibroin, and significant differences with the untreated silk pattern. Also, degummed fibroin fibers were dissolved in a calcium chloride solution, in pursuit of a more versatile material. Additionally, different porous membranes of dialysis tubes were tested to remove residual salts, and were compared by conductivity measurements and EDS analysis, identifying good performance for an affordable food cellulose membrane. The results showed that it is possible to obtain fibroin with adequate chemical properties, using low-cost process and membrane of dialysis tubes. 2020 artículo científico 0120-6230 https://www.redalyc.org/articulo.oa?id=43062873010 https://www.redalyc.org/journal/430/43062873010/ https://www.redalyc.org/journal/430/43062873010/html/ https://www.redalyc.org/journal/430/43062873010/43062873010.epub https://www.redalyc.org/journal/430/43062873010/movil 10.17533/udea.redin.20190731 en http://www.redalyc.org/revista.oa?id=430 Revista Facultad de Ingeniería Universidad de Antioquia application/pdf Universidad de Antioquia Revista Facultad de Ingeniería Universidad de Antioquia (Colombia) Num.94 |
| title | Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution |
| topic | Ingeniería dialysis membrane Silk fibroin degumming process tissue engineering |
| url | https://www.redalyc.org/articulo.oa?id=43062873010 https://www.redalyc.org/journal/430/43062873010/ https://www.redalyc.org/journal/430/43062873010/html/ https://www.redalyc.org/journal/430/43062873010/43062873010.epub https://www.redalyc.org/journal/430/43062873010/movil |