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Bibliographic Details
Main Author:	Gustavo Santoyo
Format:	Artículo científico
Language:	es
Published:	Universidad Nacional Autónoma de México 2018
Subjects:	Biología levadura Cromo hexavalente frecuencia de mutación
Online Access:	https://www.redalyc.org/articulo.oa?id=43253401004 https://www.redalyc.org/journal/432/43253401004/ https://www.redalyc.org/journal/432/43253401004/html/ https://www.redalyc.org/journal/432/43253401004/43253401004.epub https://www.redalyc.org/journal/432/43253401004/movil
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El cromo (VI) Induce la frecuencia de mutación y pérdida de Heterocigocidad En Saccharomyces cerevisiae Gustavo Santoyo Biología levadura Cromo hexavalente frecuencia de mutación El cromo (Cr) es uno de los principales contaminantes en desechos industriales, asociado con diversos daños a la saludhumana, motivo por el que en este trabajo analizamos el efecto del Cr hexavalente sobre la frecuencia de mutaciónespontánea (utilizando el gen reporteroCAN1) y la pérdida de heterocigocidad en la levaduraSaccharomycescerevisiae. Se comparó su efecto en una mutante en el genrev3, que codifica para la polimerasa zeta. Los resultadossugieren que el Cr(VI) puede inducir la frecuencia de mutación entre 20 y 28 veces, a concentraciones de 25 y 50mM, respectivamente eincrementarse en una mutanterev3y aunque no se observaron los mismos niveles como enla cepa silvestre, esto sugiere que, además derev3, existen otros factores que inducen la aparición de mutacionesen el marcador empleado. El Cr también tuvo un efecto al aumentar la pérdida de heterocigocidad de entre 50 y57 veces en la cepa silvestre y la mutanterev3, respectivamente. Estos resultados muestran queREV3participa en elefecto mutagénico causado por el Cr(VI), pero no en la pérdida de heterocigocidad, en las células de la levadura. 2018 artículo científico 1405-888X https://www.redalyc.org/articulo.oa?id=43253401004 https://www.redalyc.org/journal/432/43253401004/ https://www.redalyc.org/journal/432/43253401004/html/ https://www.redalyc.org/journal/432/43253401004/43253401004.epub https://www.redalyc.org/journal/432/43253401004/movil es http://www.redalyc.org/revista.oa?id=432 Tip Revista Especializada en Ciencias Químico-Biológicas application/pdf Universidad Nacional Autónoma de México Tip Revista Especializada en Ciencias Químico-Biológicas (México) Num.1 Vol.21

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// Anecdotal testing Today and Yesterday seems to indicate destroy() // is called by the garbage collector and everything is good. // Something to keep in mind though. return 0; } /** * Write function that is called when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ public function write($sessId, $data): bool { if ($this->writesDisabled) { return true; } return $this->saveSession($sessId, $data); } /** * A function that is called internally when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ abstract protected function saveSession($sessId, $data): bool; }

* * @return void */ public function writeClose() { // The assumption is that we're using PHP's ext/session. // session_write_close() will actually overwrite $_SESSION with an // empty array on completion -- which leads to a mismatch between what // is in the storage object and $_SESSION. To get around this, we // temporarily reset $_SESSION to an array, and then re-link it to // the storage object. // // Additionally, while you _can_ write to $_SESSION following a // session_write_close() operation, no changes made to it will be // flushed to the session handler. As such, we now mark the storage // object isImmutable. $storage = $this->getStorage(); if (! $storage->isImmutable()) { $_SESSION = $storage->toArray(true); session_write_close(); $storage->fromArray($_SESSION); $storage->markImmutable(); } } /** * Attempt to set the session name * * If the session has already been started, or if the name provided fails * validation, an exception will be raised. * * @param string $name * @return SessionManager * @throws Exception\InvalidArgumentException */ public function setName($name) { if ($this->sessionExists()) { throw new Exception\InvalidArgumentException( 'Cannot set session name after a session has already started'

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