Antibacterial activity of Streptomyces sp. Y15 against pathogenic bacteria and evaluation of culture media for antibiotic production
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| Format: | Artículo científico |
| Sprache: | en |
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Universidad Nacional Autónoma de México
2022
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| _version_ | 1876487819436752896 |
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| author | Zahaed Evangelista-Martínez |
| author_facet | Zahaed Evangelista-Martínez |
| contents | Antibacterial activity of Streptomyces sp. Y15 against pathogenic bacteria and evaluation of culture media for antibiotic production Zahaed Evangelista-Martínez Diana E. Ríos-Muñiz Jessica Gómez-Cano Ashley C. Montoya-Hidalgo Reyna E. Ochoa-Solórzano Biología antibiotics Streptomyces pathogenic bacteria secondary metabolites antimicrobial activity A notable characteristic of Streptomyces bacteria is their ability to produce secondary metabolites. The aims of this study were to identify streptomycetes that exert antibacterial activity against plant and human pathogenic bacteria, and to evaluate antibiotic production in various culture media. To identify streptomycetes that exert broad antibacterial activity using the agar-disk diffusion method, 31 isolates were screened, after which a selected isolate was cultured in nine different growth media to evaluate the production of inhibitory metabolites. Three streptomycete isolates were identified that exert inhibitory activity against at least four pathogens species. The Y15 isolate showed broad antibacterial activity on Escherichia coli, Staphylococcus epidermidis, Listeria monocytogenes, Staphylococcus aureus, Pseudomonas syringae pv. phaseolicola, Dickeya dadantii, and Pectobacterium carotovorum. The production rate of antimicrobial metabolites was maintained when Y15 was grown in culture media containing limited organic nitrogen sources. The presence of organic nitrogen compounds in the media decreased the production of antibacterial metabolites. The Y15 isolate was identified by 16S rDNA gene sequencing as member of the genus Streptomyces. We show that the composition of the fermentation medium is likely an important factor for modulating antibiotic production by streptomycetes, with impacts on the quantity and diversity of antimicrobial metabolites production. 2022 artículo científico 1405-888X https://www.redalyc.org/articulo.oa?id=43275501002 https://www.redalyc.org/journal/432/43275501002/ https://www.redalyc.org/journal/432/43275501002/html/ https://www.redalyc.org/journal/432/43275501002/43275501002.epub https://www.redalyc.org/journal/432/43275501002/movil https://doi.org/10.22201/fesz.23958723e.2022.415 en http://www.redalyc.org/revista.oa?id=432 Tip Revista Especializada en Ciencias Químico-Biológicas application/pdf Universidad Nacional Autónoma de México Tip Revista Especializada en Ciencias Químico-Biológicas (México) Vol.25 |
| format | Artículo científico |
| id | redalyc_43275501002 |
| institution | Redalyc |
| language | en |
| publishDate | 2022 |
| publisher | Universidad Nacional Autónoma de México |
| spellingShingle | Antibacterial activity of Streptomyces sp. Y15 against pathogenic bacteria and evaluation of culture media for antibiotic production Zahaed Evangelista-Martínez Biología antibiotics Streptomyces pathogenic bacteria secondary metabolites antimicrobial activity Antibacterial activity of Streptomyces sp. Y15 against pathogenic bacteria and evaluation of culture media for antibiotic production Zahaed Evangelista-Martínez Diana E. Ríos-Muñiz Jessica Gómez-Cano Ashley C. Montoya-Hidalgo Reyna E. Ochoa-Solórzano Biología antibiotics Streptomyces pathogenic bacteria secondary metabolites antimicrobial activity A notable characteristic of Streptomyces bacteria is their ability to produce secondary metabolites. The aims of this study were to identify streptomycetes that exert antibacterial activity against plant and human pathogenic bacteria, and to evaluate antibiotic production in various culture media. To identify streptomycetes that exert broad antibacterial activity using the agar-disk diffusion method, 31 isolates were screened, after which a selected isolate was cultured in nine different growth media to evaluate the production of inhibitory metabolites. Three streptomycete isolates were identified that exert inhibitory activity against at least four pathogens species. The Y15 isolate showed broad antibacterial activity on Escherichia coli, Staphylococcus epidermidis, Listeria monocytogenes, Staphylococcus aureus, Pseudomonas syringae pv. phaseolicola, Dickeya dadantii, and Pectobacterium carotovorum. The production rate of antimicrobial metabolites was maintained when Y15 was grown in culture media containing limited organic nitrogen sources. The presence of organic nitrogen compounds in the media decreased the production of antibacterial metabolites. The Y15 isolate was identified by 16S rDNA gene sequencing as member of the genus Streptomyces. We show that the composition of the fermentation medium is likely an important factor for modulating antibiotic production by streptomycetes, with impacts on the quantity and diversity of antimicrobial metabolites production. 2022 artículo científico 1405-888X https://www.redalyc.org/articulo.oa?id=43275501002 https://www.redalyc.org/journal/432/43275501002/ https://www.redalyc.org/journal/432/43275501002/html/ https://www.redalyc.org/journal/432/43275501002/43275501002.epub https://www.redalyc.org/journal/432/43275501002/movil https://doi.org/10.22201/fesz.23958723e.2022.415 en http://www.redalyc.org/revista.oa?id=432 Tip Revista Especializada en Ciencias Químico-Biológicas application/pdf Universidad Nacional Autónoma de México Tip Revista Especializada en Ciencias Químico-Biológicas (México) Vol.25 |
| title | Antibacterial activity of Streptomyces sp. Y15 against pathogenic bacteria and evaluation of culture media for antibiotic production |
| topic | Biología antibiotics Streptomyces pathogenic bacteria secondary metabolites antimicrobial activity |
| url | https://www.redalyc.org/articulo.oa?id=43275501002 https://www.redalyc.org/journal/432/43275501002/ https://www.redalyc.org/journal/432/43275501002/html/ https://www.redalyc.org/journal/432/43275501002/43275501002.epub https://www.redalyc.org/journal/432/43275501002/movil https://doi.org/10.22201/fesz.23958723e.2022.415 |