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| Format: | Artículo científico |
| Language: | en |
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Universidad de Costa Rica
2008
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| Online Access: | https://www.redalyc.org/articulo.oa?id=43632113 |
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Table of Contents:
- Direct somatic embryogenesis in coffea arabica L. CVS. caturra and catuaí: effect of triacontanol, light condition, and medium consistency Andrés M. Gatica Griselda Arrieta Ana M. Espinoza Agrociencias Coffee darkness tissue culture semisolid medium plant growth regulators The influence of triacontanol (TRIA)concentration and its interaction with indole-3-acetic acid (IAA) on direct somatic embryogenesis(DSE) of Coffea arabica L. cvs Caturra andCatuaí was determined. Additionally, lightconditions and culture medium consistency(semisolid vs. liquid) were evaluated. A higheraverage of somatic embryos per explant fromCaturra (3.9±0.5) and Catuaí (3.6±0.5) leaveswas obtained when 4.55 µM TRIA was addedto the half-strength Murashige and Skoog(1962) medium supplemented with 1.1 µMbenzilaminopurine (BAP) and 2.85 µM IAA.In relation to medium consistency, the highestnumber of embryoids in Caturra (3.2±0.2)and Catuaí (6.0±0.4) explants was obtainedusing Yasuda et al. (1985) semisolid medium.Regarding photoperiod, the number of embryoidsper explant obtained for Catuaí cultured under a16 h light photoperiod was 7.6±1.0 and in thedark 6.2±0.6. In Caturra, 4.2±0.4 embryoidswere produced in the dark and 3.8±0.5 with 16h light. No somatic embryos were observed inCaturra and Catuaí explants after 12 weeks ofculture in liquid Yasuda et al. (1985) medium,and under any of the light conditions. 2008 artículo científico 0377-9424 https://www.redalyc.org/articulo.oa?id=43632113 en http://www.redalyc.org/revista.oa?id=436 Agronomía Costarricense application/pdf Universidad de Costa Rica Agronomía Costarricense (Costa Rica) Num.1 Vol.32