Erwinia billingiae causes bacterial Canker of Mango (Mangifera indica) in Costa Rica
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| Format: | Artículo científico |
| Language: | en |
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Universidad de Costa Rica
2021
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| _version_ | 1876488430727200768 |
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| author | Daniela Vidaurre-Barahona |
| author_facet | Daniela Vidaurre-Barahona |
| contents | Erwinia billingiae causes bacterial Canker of Mango (Mangifera indica) in Costa Rica Daniela Vidaurre-Barahona Amy Wang-Wong Lorena Uribe-Lorío Agrociencias Tommy Atkins Erwinia mangifera Erwinia herbicola Enterobacteriaceae Introduction. In Costa Rica, bacterial canker of mango has caused economic losses in most of the productive areas since the mid-1980s. The causal agents have been identified only by phenotypic methods such as Erwinia mangifera and E. herbicola. Objective. To confirm, using a molecular and phenotypic approach, the species of the Enterobacteriaceae the cause bacterial canker of mango in Costa Rica. Material and methods. Fruits, branches, and trunks with symptoms were collected in different orchards in the Alajuela province. Bacterial isolation was performed, and pathogenicity was evaluated by inoculating fruits and trunks of the Tommy Atkins variety. The positive isolates for the pathogenic test were re-inoculated, isolated, and identified in order to fulfill Koch’s postulates. The CIBCM-Mg-115 positive isolate that caused symptoms was analyzed by complete biochemical characterization and molecular identification by phylogenetic analyses of 16S rRNA and the atpD, gyrB, infB, and rpoB housekeeping genes. Results. According to the data obtained from the biochemical and molecular analysis, the CIBCM-Mg-115 strain was identified as Erwinia billingiae. Conclusion. E. billingiae corresponds to one of the causal agents of bacterial canker on mango (M. indica) trees in Costa Rica. 2021 artículo científico 2215-3608 https://www.redalyc.org/articulo.oa?id=43765068022 https://www.redalyc.org/journal/437/43765068022/ https://www.redalyc.org/journal/437/43765068022/html/ https://www.redalyc.org/journal/437/43765068022/43765068022.epub https://www.redalyc.org/journal/437/43765068022/movil https://doi.org/10.15517/am.v32i1.40965 en http://www.redalyc.org/revista.oa?id=437 Agronomía Mesoamericana application/pdf Universidad de Costa Rica Agronomía Mesoamericana (Costa Rica) Num.1 Vol.32 |
| format | Artículo científico |
| id | redalyc_43765068022 |
| institution | Redalyc |
| language | en |
| publishDate | 2021 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Erwinia billingiae causes bacterial Canker of Mango (Mangifera indica) in Costa Rica Daniela Vidaurre-Barahona Agrociencias Tommy Atkins Erwinia mangifera Erwinia herbicola Enterobacteriaceae Erwinia billingiae causes bacterial Canker of Mango (Mangifera indica) in Costa Rica Daniela Vidaurre-Barahona Amy Wang-Wong Lorena Uribe-Lorío Agrociencias Tommy Atkins Erwinia mangifera Erwinia herbicola Enterobacteriaceae Introduction. In Costa Rica, bacterial canker of mango has caused economic losses in most of the productive areas since the mid-1980s. The causal agents have been identified only by phenotypic methods such as Erwinia mangifera and E. herbicola. Objective. To confirm, using a molecular and phenotypic approach, the species of the Enterobacteriaceae the cause bacterial canker of mango in Costa Rica. Material and methods. Fruits, branches, and trunks with symptoms were collected in different orchards in the Alajuela province. Bacterial isolation was performed, and pathogenicity was evaluated by inoculating fruits and trunks of the Tommy Atkins variety. The positive isolates for the pathogenic test were re-inoculated, isolated, and identified in order to fulfill Koch’s postulates. The CIBCM-Mg-115 positive isolate that caused symptoms was analyzed by complete biochemical characterization and molecular identification by phylogenetic analyses of 16S rRNA and the atpD, gyrB, infB, and rpoB housekeeping genes. Results. According to the data obtained from the biochemical and molecular analysis, the CIBCM-Mg-115 strain was identified as Erwinia billingiae. Conclusion. E. billingiae corresponds to one of the causal agents of bacterial canker on mango (M. indica) trees in Costa Rica. 2021 artículo científico 2215-3608 https://www.redalyc.org/articulo.oa?id=43765068022 https://www.redalyc.org/journal/437/43765068022/ https://www.redalyc.org/journal/437/43765068022/html/ https://www.redalyc.org/journal/437/43765068022/43765068022.epub https://www.redalyc.org/journal/437/43765068022/movil https://doi.org/10.15517/am.v32i1.40965 en http://www.redalyc.org/revista.oa?id=437 Agronomía Mesoamericana application/pdf Universidad de Costa Rica Agronomía Mesoamericana (Costa Rica) Num.1 Vol.32 |
| title | Erwinia billingiae causes bacterial Canker of Mango (Mangifera indica) in Costa Rica |
| topic | Agrociencias Tommy Atkins Erwinia mangifera Erwinia herbicola Enterobacteriaceae |
| url | https://www.redalyc.org/articulo.oa?id=43765068022 https://www.redalyc.org/journal/437/43765068022/ https://www.redalyc.org/journal/437/43765068022/html/ https://www.redalyc.org/journal/437/43765068022/43765068022.epub https://www.redalyc.org/journal/437/43765068022/movil https://doi.org/10.15517/am.v32i1.40965 |