Soybean leaf infusion culture media and phytotoxic metabolite production in Macrophomina phaseolina
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidad de Costa Rica
2025
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| _version_ | 1876471094422011904 |
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| author | Jazmín Vaceque-Acosta |
| author_facet | Jazmín Vaceque-Acosta |
| contents | Soybean leaf infusion culture media and phytotoxic metabolite production in Macrophomina phaseolina Jazmín Vaceque-Acosta Javier E. Barúa Dani Daniel Ruiz-Diaz-Mendoza M. Cristina Romero-Rodríguez Antonio Macías-Sánchez María Eugenia Flores-Giubi Agrociencias charcoal rot bioactive molecules Fungal phytopathogen secondary metabolites Introduction. The charcoal rot fungus, Macrophomina phaseolina, is a ubiquitous necrotrophic phytopathogen that infecting soybean and other plant species. Despite its significant impact on crops, limited progress has been made in understanding the factors that influence phytotoxic molecule secretion by this phytopathogen. Objective. To evaluate the effect of soybean leaf infusion in the culture medium on the differential secretion of phytotoxic molecules of M. phaseolina. Materials and methods. The study was conducted between 2016 and 2023 at the Departamento de Química Biológica, Universidad Nacional de Asunción, Paraguay. Two fungal isolates were cultured in vitro using potato dextrose broth (PDB) and Czapek-Dox broth media, with or without soybean leaf infusion. Phytotoxic activity of secreted molecules was a using soybean leaf discs. The crude organic extract from the cultures was separated using chromatographic techniques, and purified metabolites were characterized by UHPLC-PDA/MS, HRMS (APGC), HRMSESI, 1HNMR and 13CNMR. Results. Molecules secreted by M. phaseolina FCQ11 cultured in infusion-enriched PDB induced the highest percentage of necrosis. Under these conditions, three differentially secreted metabolites were isolated and identified: (R)-mellein, (3R,4R)-hydroxymellein, and (-)-botryodiplodin. Conclusions. Soybean leaf infusion presence in M. phaseolina growth media stimulates phytotoxic metabolite production and alters the profile of secreted metabolites. 2025 artículo científico 2215-3608 https://www.redalyc.org/articulo.oa?id=43780398050 https://www.redalyc.org/journal/437/43780398050/ https://www.redalyc.org/journal/437/43780398050/html/ https://www.redalyc.org/journal/437/43780398050/43780398050.epub https://www.redalyc.org/journal/437/43780398050/movil 10.15517/x1y07135 en http://www.redalyc.org/revista.oa?id=437 Agronomía Mesoamericana application/pdf Universidad de Costa Rica Agronomía Mesoamericana (Costa Rica) Vol.36 |
| format | Artículo científico |
| id | redalyc_43780398050 |
| institution | Redalyc |
| language | en |
| publishDate | 2025 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Soybean leaf infusion culture media and phytotoxic metabolite production in Macrophomina phaseolina Jazmín Vaceque-Acosta Agrociencias charcoal rot bioactive molecules Fungal phytopathogen secondary metabolites Soybean leaf infusion culture media and phytotoxic metabolite production in Macrophomina phaseolina Jazmín Vaceque-Acosta Javier E. Barúa Dani Daniel Ruiz-Diaz-Mendoza M. Cristina Romero-Rodríguez Antonio Macías-Sánchez María Eugenia Flores-Giubi Agrociencias charcoal rot bioactive molecules Fungal phytopathogen secondary metabolites Introduction. The charcoal rot fungus, Macrophomina phaseolina, is a ubiquitous necrotrophic phytopathogen that infecting soybean and other plant species. Despite its significant impact on crops, limited progress has been made in understanding the factors that influence phytotoxic molecule secretion by this phytopathogen. Objective. To evaluate the effect of soybean leaf infusion in the culture medium on the differential secretion of phytotoxic molecules of M. phaseolina. Materials and methods. The study was conducted between 2016 and 2023 at the Departamento de Química Biológica, Universidad Nacional de Asunción, Paraguay. Two fungal isolates were cultured in vitro using potato dextrose broth (PDB) and Czapek-Dox broth media, with or without soybean leaf infusion. Phytotoxic activity of secreted molecules was a using soybean leaf discs. The crude organic extract from the cultures was separated using chromatographic techniques, and purified metabolites were characterized by UHPLC-PDA/MS, HRMS (APGC), HRMSESI, 1HNMR and 13CNMR. Results. Molecules secreted by M. phaseolina FCQ11 cultured in infusion-enriched PDB induced the highest percentage of necrosis. Under these conditions, three differentially secreted metabolites were isolated and identified: (R)-mellein, (3R,4R)-hydroxymellein, and (-)-botryodiplodin. Conclusions. Soybean leaf infusion presence in M. phaseolina growth media stimulates phytotoxic metabolite production and alters the profile of secreted metabolites. 2025 artículo científico 2215-3608 https://www.redalyc.org/articulo.oa?id=43780398050 https://www.redalyc.org/journal/437/43780398050/ https://www.redalyc.org/journal/437/43780398050/html/ https://www.redalyc.org/journal/437/43780398050/43780398050.epub https://www.redalyc.org/journal/437/43780398050/movil 10.15517/x1y07135 en http://www.redalyc.org/revista.oa?id=437 Agronomía Mesoamericana application/pdf Universidad de Costa Rica Agronomía Mesoamericana (Costa Rica) Vol.36 |
| title | Soybean leaf infusion culture media and phytotoxic metabolite production in Macrophomina phaseolina |
| topic | Agrociencias charcoal rot bioactive molecules Fungal phytopathogen secondary metabolites |
| url | https://www.redalyc.org/articulo.oa?id=43780398050 https://www.redalyc.org/journal/437/43780398050/ https://www.redalyc.org/journal/437/43780398050/html/ https://www.redalyc.org/journal/437/43780398050/43780398050.epub https://www.redalyc.org/journal/437/43780398050/movil |