Effect of Temperature-Time on the Sterilization Process for a Jacketed Bioreactor System: Application of a Ratkowsky Nonlinear Model
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidad de Costa Rica
2018
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| _version_ | 1876457083354742784 |
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| author | Jéssica Montero-Zamora |
| author_facet | Jéssica Montero-Zamora |
| contents | Effect of Temperature-Time on the Sterilization Process for a Jacketed Bioreactor System: Application of a Ratkowsky Nonlinear Model Jéssica Montero-Zamora Iray Mata Araya Manuel Molina Córdoba Francisco Aguilar Cascante Ingeniería aseptic autoclaving fermentation heat transfer Bioengineering Steam sterilization is a technique widely implemented in different biotechnological processes -among them the growth of microorganisms in bioreactors-, which require initial aseptic conditions. In the present research, the relationship between lethality and death rate of Bacillus cereus grown in 7-liter stirred-tank jacketed bioreactors was investigated, with sugar cane molasses as a carbon source. The sterilization process was tested with an industrial autoclave within data loggers in bioreactors, which measure the temperature in the cold point to quantify the accumulated lethality. Through data analysis, a contour plot allows a graphical prediction of the microbiological sterilization results in terms of colony forming units (CFU). The study shows that there is a range between 16 and 20 minutes, approaching 123 °C, with a null presence of contaminant microorganisms. The surface chart demonstrates the existing non-linear relationship between the variables temperature and time involved. A positive correlation was observed using a Ratkowsky mathematical model with a 0,971 correlation coefficient and the estimated value of α = 11,04 and β = -1,49 for the non-linear model of parameterization. With these results, it is possible to predict the CFU based on F_0 data. This could provide an interesting basis for future sterilization practices and a methodology as a starting point for sterilization trials in industry and save time and costs. 2018 artículo científico 1409-2441 https://www.redalyc.org/articulo.oa?id=44170541008 en http://www.redalyc.org/revista.oa?id=441 Ingeniería. Revista de la Universidad de Costa Rica application/pdf Universidad de Costa Rica Ingeniería. Revista de la Universidad de Costa Rica (Costa Rica) Num.2 Vol.28 |
| format | Artículo científico |
| id | redalyc_44170541008 |
| institution | Redalyc |
| language | en |
| publishDate | 2018 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Effect of Temperature-Time on the Sterilization Process for a Jacketed Bioreactor System: Application of a Ratkowsky Nonlinear Model Jéssica Montero-Zamora Ingeniería aseptic autoclaving fermentation heat transfer Bioengineering Effect of Temperature-Time on the Sterilization Process for a Jacketed Bioreactor System: Application of a Ratkowsky Nonlinear Model Jéssica Montero-Zamora Iray Mata Araya Manuel Molina Córdoba Francisco Aguilar Cascante Ingeniería aseptic autoclaving fermentation heat transfer Bioengineering Steam sterilization is a technique widely implemented in different biotechnological processes -among them the growth of microorganisms in bioreactors-, which require initial aseptic conditions. In the present research, the relationship between lethality and death rate of Bacillus cereus grown in 7-liter stirred-tank jacketed bioreactors was investigated, with sugar cane molasses as a carbon source. The sterilization process was tested with an industrial autoclave within data loggers in bioreactors, which measure the temperature in the cold point to quantify the accumulated lethality. Through data analysis, a contour plot allows a graphical prediction of the microbiological sterilization results in terms of colony forming units (CFU). The study shows that there is a range between 16 and 20 minutes, approaching 123 °C, with a null presence of contaminant microorganisms. The surface chart demonstrates the existing non-linear relationship between the variables temperature and time involved. A positive correlation was observed using a Ratkowsky mathematical model with a 0,971 correlation coefficient and the estimated value of α = 11,04 and β = -1,49 for the non-linear model of parameterization. With these results, it is possible to predict the CFU based on F_0 data. This could provide an interesting basis for future sterilization practices and a methodology as a starting point for sterilization trials in industry and save time and costs. 2018 artículo científico 1409-2441 https://www.redalyc.org/articulo.oa?id=44170541008 en http://www.redalyc.org/revista.oa?id=441 Ingeniería. Revista de la Universidad de Costa Rica application/pdf Universidad de Costa Rica Ingeniería. Revista de la Universidad de Costa Rica (Costa Rica) Num.2 Vol.28 |
| title | Effect of Temperature-Time on the Sterilization Process for a Jacketed Bioreactor System: Application of a Ratkowsky Nonlinear Model |
| topic | Ingeniería aseptic autoclaving fermentation heat transfer Bioengineering |
| url | https://www.redalyc.org/articulo.oa?id=44170541008 |