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| Format: | Artículo científico |
| Language: | es |
| Published: |
Universidad de Costa Rica
2007
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| Online Access: | https://www.redalyc.org/articulo.oa?id=44339813081 |
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Table of Contents:
- PROPAGACIÓN IN VITRO Y ACLIMATIZACIÓN DE EUCHILE MARIAE (AMES) WITHNER (ORCHIDACEAE) IRIS SUÁREZ-QUIJADA MABEL HERNÁNDEZ-ALTAMIRANO VÍCTOR M. CHÁVEZ-ÁVILA ESTELA SANDOVAL-ZAPOTITLA ALEJANDRO MARTINEZ-PALACIOS Biología Orchid micropropagation of species Euchile mariae was achieved from the in vitro culture of protocorms sections, obtained from the germination of seeds. The top and bottom protocorms sections, used as explants, were cultivated in modified MS culture medium, added with different concentrations of a-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BA). The morphogenetic response obtained of both types of explants, was the formation of protocorm like bodies (PLB’s), that gave place to the formation of new plantlets. Of two tested explants the biggest formation of PLB’s was obtained from the bottom protocorms sections. The acclimatization of the plantlets was successful, with a 100% percent survival. Plant cell tissue culture represents a useful alternative for the study, conservation and massive propagation of this and other endangered species. 2007 artículo científico 1409-3871 https://www.redalyc.org/articulo.oa?id=44339813081 es http://www.redalyc.org/revista.oa?id=443 Lankesteriana International Journal on Orchidology application/pdf Universidad de Costa Rica Lankesteriana International Journal on Orchidology (Costa Rica) Num.1-2 Vol.7