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| Format: | Artículo científico |
| Language: | en |
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Universidad de Costa Rica
2011
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| Online Access: | https://www.redalyc.org/articulo.oa?id=44339820003 |
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| _version_ | 1866811700780466176 |
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| author | María Valdés |
| author_facet | María Valdés |
| contents | The root colonizing fungi of the terrestrial orchid Cypripedium irapeanum María Valdés Héctor Bautista Guerrero Laura Martínez Rafael H. Víquez Biología microsclerotia Terrestrial orchid orchidoide mycorrhiza dark septate endophytes This study investigated the mycorrhizal status and the identification of the fungi colonizing the roots of the terrestrial orchid Cypripedium irapeanu by restriction fragment length polymorphisms and by rDNA internal transcribed spacer sequencing. The orchid is endemic of differents regions in Mexico, Guatemala and Honduras; usually at 1400-2250 m. It grows mainly in the remaining oak forests of the highlands and it is in the Mexican red list of plants in danger. The oak forests in Mexico are threatened constantly. The microscopic examination of stained root segments of the orchid revealed the presence of fungal structures of both orchidioid fungi (pelotons and coyled hyphae) and dark septate endophytes (DSE) (mielinized hyphae and microsclerotia). Analysis of ITS1-5.8-ITS2 region sequences suggested that mycorrhizal tissue was dominated by Tulasnaceae: Sistotrema sp., Rhizoctonia solani, and Epulorhiza sp. Among the DSE one isolate revealed 100% similarity to Phomopsis sp XJ-05, and another one 99% to the fungal endophyte MUT 885 which are both reported as dark septate endophytes. The putative dark septate endophyte Phomopsis sp XJ-05 was isolated not only from the roots but also from the germinated seeds of C. irapeanum 2011 artículo científico 1409-3871 https://www.redalyc.org/articulo.oa?id=44339820003 en http://www.redalyc.org/revista.oa?id=443 Lankesteriana International Journal on Orchidology application/pdf Universidad de Costa Rica Lankesteriana International Journal on Orchidology (Costa Rica) Num.1 Vol.11 |
| format | Artículo científico |
| id | redalyc_44339820003 |
| language | en |
| publishDate | 2011 |
| publisher | Universidad de Costa Rica |
| spellingShingle | The root colonizing fungi of the terrestrial orchid Cypripedium irapeanum María Valdés Biología microsclerotia Terrestrial orchid orchidoide mycorrhiza dark septate endophytes The root colonizing fungi of the terrestrial orchid Cypripedium irapeanum María Valdés Héctor Bautista Guerrero Laura Martínez Rafael H. Víquez Biología microsclerotia Terrestrial orchid orchidoide mycorrhiza dark septate endophytes This study investigated the mycorrhizal status and the identification of the fungi colonizing the roots of the terrestrial orchid Cypripedium irapeanu by restriction fragment length polymorphisms and by rDNA internal transcribed spacer sequencing. The orchid is endemic of differents regions in Mexico, Guatemala and Honduras; usually at 1400-2250 m. It grows mainly in the remaining oak forests of the highlands and it is in the Mexican red list of plants in danger. The oak forests in Mexico are threatened constantly. The microscopic examination of stained root segments of the orchid revealed the presence of fungal structures of both orchidioid fungi (pelotons and coyled hyphae) and dark septate endophytes (DSE) (mielinized hyphae and microsclerotia). Analysis of ITS1-5.8-ITS2 region sequences suggested that mycorrhizal tissue was dominated by Tulasnaceae: Sistotrema sp., Rhizoctonia solani, and Epulorhiza sp. Among the DSE one isolate revealed 100% similarity to Phomopsis sp XJ-05, and another one 99% to the fungal endophyte MUT 885 which are both reported as dark septate endophytes. The putative dark septate endophyte Phomopsis sp XJ-05 was isolated not only from the roots but also from the germinated seeds of C. irapeanum 2011 artículo científico 1409-3871 https://www.redalyc.org/articulo.oa?id=44339820003 en http://www.redalyc.org/revista.oa?id=443 Lankesteriana International Journal on Orchidology application/pdf Universidad de Costa Rica Lankesteriana International Journal on Orchidology (Costa Rica) Num.1 Vol.11 |
| title | The root colonizing fungi of the terrestrial orchid Cypripedium irapeanum |
| topic | Biología microsclerotia Terrestrial orchid orchidoide mycorrhiza dark septate endophytes |
| url | https://www.redalyc.org/articulo.oa?id=44339820003 |