Extraction and amplification of DNA from orchid exsiccates conserved for more than half a century in a herbarium in Bogotá, Colom
Fuente:
Redalyc
Saved in:
| Main Author: | |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad de Costa Rica
2012
|
| Subjects: | |
| Online Access: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1876488026865008640 |
|---|---|
| author | Laura C. Mazo |
| author_facet | Laura C. Mazo |
| contents | Extraction and amplification of DNA from orchid exsiccates conserved for more than half a century in a herbarium in Bogotá, Colom Laura C. Mazo Alberto Gómez Sonia R. Quintanilla Jaime E. Bernal Pedro Ortiz Valdivieso Biología DNA DNA aDNA aDNA matK Plant tissue from herbarium specimens contains DNA that has undergone post-mortem degradation. Only small amounts of possibly degraded genetic material free of chemicals and impurities can be extracted from these samples. The aim of the present work was to compare and determine which one of three previously published plant DNA extraction protocols would extract good quality DNA from orchid herbarium specimens stored up to 63 years, susceptible of PCR amplification and Sanger sequencing. The most effective protocol for this type of samples allowed us to obtain and sequence amplified products in 6 of the 7 samples studied with an optimal DNA / protein purity relationship. 2012 artículo científico 1409-3871 https://www.redalyc.org/articulo.oa?id=44339824005 en http://www.redalyc.org/revista.oa?id=443 Lankesteriana International Journal on Orchidology application/pdf Universidad de Costa Rica Lankesteriana International Journal on Orchidology (Costa Rica) Num.2 Vol.12 |
| format | Artículo científico |
| id | redalyc_44339824005 |
| institution | Redalyc |
| language | en |
| publishDate | 2012 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Extraction and amplification of DNA from orchid exsiccates conserved for more than half a century in a herbarium in Bogotá, Colom Laura C. Mazo Biología DNA DNA aDNA aDNA matK Extraction and amplification of DNA from orchid exsiccates conserved for more than half a century in a herbarium in Bogotá, Colom Laura C. Mazo Alberto Gómez Sonia R. Quintanilla Jaime E. Bernal Pedro Ortiz Valdivieso Biología DNA DNA aDNA aDNA matK Plant tissue from herbarium specimens contains DNA that has undergone post-mortem degradation. Only small amounts of possibly degraded genetic material free of chemicals and impurities can be extracted from these samples. The aim of the present work was to compare and determine which one of three previously published plant DNA extraction protocols would extract good quality DNA from orchid herbarium specimens stored up to 63 years, susceptible of PCR amplification and Sanger sequencing. The most effective protocol for this type of samples allowed us to obtain and sequence amplified products in 6 of the 7 samples studied with an optimal DNA / protein purity relationship. 2012 artículo científico 1409-3871 https://www.redalyc.org/articulo.oa?id=44339824005 en http://www.redalyc.org/revista.oa?id=443 Lankesteriana International Journal on Orchidology application/pdf Universidad de Costa Rica Lankesteriana International Journal on Orchidology (Costa Rica) Num.2 Vol.12 |
| title | Extraction and amplification of DNA from orchid exsiccates conserved for more than half a century in a herbarium in Bogotá, Colom |
| topic | Biología DNA DNA aDNA aDNA matK |
| url | https://www.redalyc.org/articulo.oa?id=44339824005 |