PROPAGATION OF DENDROBIUM AGGREGATUM BY GREEN CAPSULE CULTURE
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Universidad de Costa Rica
2012
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| _version_ | 1876477525380562944 |
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| author | S. Vijayakumar |
| author_facet | S. Vijayakumar |
| contents | PROPAGATION OF DENDROBIUM AGGREGATUM BY GREEN CAPSULE CULTURE S. Vijayakumar G. Rajalkshmi K. Kalimuthu Biología in vitro Dendrobium acclimatization protocorm like bodies An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month. 2012 artículo científico 1409-3871 https://www.redalyc.org/articulo.oa?id=44339824006 en http://www.redalyc.org/revista.oa?id=443 Lankesteriana International Journal on Orchidology application/pdf Universidad de Costa Rica Lankesteriana International Journal on Orchidology (Costa Rica) Num.2 Vol.12 |
| format | Artículo científico |
| id | redalyc_44339824006 |
| institution | Redalyc |
| language | en |
| publishDate | 2012 |
| publisher | Universidad de Costa Rica |
| spellingShingle | PROPAGATION OF DENDROBIUM AGGREGATUM BY GREEN CAPSULE CULTURE S. Vijayakumar Biología in vitro Dendrobium acclimatization protocorm like bodies PROPAGATION OF DENDROBIUM AGGREGATUM BY GREEN CAPSULE CULTURE S. Vijayakumar G. Rajalkshmi K. Kalimuthu Biología in vitro Dendrobium acclimatization protocorm like bodies An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month. 2012 artículo científico 1409-3871 https://www.redalyc.org/articulo.oa?id=44339824006 en http://www.redalyc.org/revista.oa?id=443 Lankesteriana International Journal on Orchidology application/pdf Universidad de Costa Rica Lankesteriana International Journal on Orchidology (Costa Rica) Num.2 Vol.12 |
| title | PROPAGATION OF DENDROBIUM AGGREGATUM BY GREEN CAPSULE CULTURE |
| topic | Biología in vitro Dendrobium acclimatization protocorm like bodies |
| url | https://www.redalyc.org/articulo.oa?id=44339824006 |