CALLUS GROWTH AND PLANT REGENERATION IN LAELIA SPECIOSA (ORCHIDACEAE)
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| Format: | Artículo científico |
| Sprache: | en |
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Universidad de Costa Rica
2010
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| author | Marcela Esmeralda Sarabia-Ochoa |
| author_facet | Marcela Esmeralda Sarabia-Ochoa |
| contents | CALLUS GROWTH AND PLANT REGENERATION IN LAELIA SPECIOSA (ORCHIDACEAE) Marcela Esmeralda Sarabia-Ochoa Irene Avila-Díaz Alonso Carlos-Gómez Rafael Salgado-Garciglia Biología callus Laelia speciosa endangered orchid plant regeneration Laelia speciosa is an endangered epiphytic orchid, endemic to México. It is thought that the asexual reproduction in vitro could be one of the actions to counteract the massive extraction of individuals from their natural populations. The growth and differentiation of callus tissues derived from leaf explants of L. speciosa were investigated in Murashige and Skoog medium (MS) with 30 g l -1 sucrose and five concentrations (0.0, 0.25, 0.5, 1.0, and 2.5 g l -1 ) of naphthaleneacetic acid (NAA) with benzyladenine (BA) (0.0, 0.25, 0.5, 1.0, and 2.5 g l -1 ). Leaf explants from in vitro plantlets formed callus tissue on MS medium supplemented with 2.5 mg l -1 BA while mature leaves did not respond. Diameter of the callus tissues averaged 1.25 cm after eight weeks of culture. PLBs development was achieved on MS medium supplemented with 2.5 mg l -1 NAA and 1 mg l -1 BA. The formation of plantlets was successfully obtained in MS supplemented with 0.5 mg l -1 of NAA and 0.1 mg l -1 of gibberellic acid (GA 3 ). Obtained plantlets were successfully acclimatized in a greenhouse with a survival rate of 70%. 2010 artículo científico 1409-3871 https://www.redalyc.org/articulo.oa?id=44340042002 en http://www.redalyc.org/revista.oa?id=443 Lankesteriana International Journal on Orchidology application/pdf Universidad de Costa Rica Lankesteriana International Journal on Orchidology (Costa Rica) Num.1 Vol.10 |
| format | Artículo científico |
| id | redalyc_44340042002 |
| language | en |
| publishDate | 2010 |
| publisher | Universidad de Costa Rica |
| spellingShingle | CALLUS GROWTH AND PLANT REGENERATION IN LAELIA SPECIOSA (ORCHIDACEAE) Marcela Esmeralda Sarabia-Ochoa Biología callus Laelia speciosa endangered orchid plant regeneration CALLUS GROWTH AND PLANT REGENERATION IN LAELIA SPECIOSA (ORCHIDACEAE) Marcela Esmeralda Sarabia-Ochoa Irene Avila-Díaz Alonso Carlos-Gómez Rafael Salgado-Garciglia Biología callus Laelia speciosa endangered orchid plant regeneration Laelia speciosa is an endangered epiphytic orchid, endemic to México. It is thought that the asexual reproduction in vitro could be one of the actions to counteract the massive extraction of individuals from their natural populations. The growth and differentiation of callus tissues derived from leaf explants of L. speciosa were investigated in Murashige and Skoog medium (MS) with 30 g l -1 sucrose and five concentrations (0.0, 0.25, 0.5, 1.0, and 2.5 g l -1 ) of naphthaleneacetic acid (NAA) with benzyladenine (BA) (0.0, 0.25, 0.5, 1.0, and 2.5 g l -1 ). Leaf explants from in vitro plantlets formed callus tissue on MS medium supplemented with 2.5 mg l -1 BA while mature leaves did not respond. Diameter of the callus tissues averaged 1.25 cm after eight weeks of culture. PLBs development was achieved on MS medium supplemented with 2.5 mg l -1 NAA and 1 mg l -1 BA. The formation of plantlets was successfully obtained in MS supplemented with 0.5 mg l -1 of NAA and 0.1 mg l -1 of gibberellic acid (GA 3 ). Obtained plantlets were successfully acclimatized in a greenhouse with a survival rate of 70%. 2010 artículo científico 1409-3871 https://www.redalyc.org/articulo.oa?id=44340042002 en http://www.redalyc.org/revista.oa?id=443 Lankesteriana International Journal on Orchidology application/pdf Universidad de Costa Rica Lankesteriana International Journal on Orchidology (Costa Rica) Num.1 Vol.10 |
| title | CALLUS GROWTH AND PLANT REGENERATION IN LAELIA SPECIOSA (ORCHIDACEAE) |
| topic | Biología callus Laelia speciosa endangered orchid plant regeneration |
| url | https://www.redalyc.org/articulo.oa?id=44340042002 |