TULASNELLA IRREGULARIS (BASIDIOMYCOTA: TULASNELLACEAE) FROM ROOTS OF ENCYCLIA TAMPENSIS IN SOUTH FLORIDA, AND CONFIRMATION OF ITS MYCORRHIZAL SIGNIFICANCE THROUGH SYMBIOTIC SEED GERMINATION
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Universidad de Costa Rica
2013
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| _version_ | 1876491277324779520 |
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| author | Lawawrence W. Zettler |
| author_facet | Lawawrence W. Zettler |
| contents | TULASNELLA IRREGULARIS (BASIDIOMYCOTA: TULASNELLACEAE) FROM ROOTS OF ENCYCLIA TAMPENSIS IN SOUTH FLORIDA, AND CONFIRMATION OF ITS MYCORRHIZAL SIGNIFICANCE THROUGH SYMBIOTIC SEED GERMINATION Lawawrence W. Zettler Laura L. Corey Alishia L. Jacks Luke T. Gruender Alyssa M. Lopez Biología physiology Epidendrum Conservation Basidiomycota ITS sequencing Epiphytic orchids remain understudied with respect to their obligate mycorrhizal relationships – a key component of the integrated conservation model. Existing studies have revealed that these plants, like their terrestrial counterparts, commonly associate with ubiquitous basidiomycetes (e.g., Tulasnellaceae); however, few studies have verified their physiological role(s). Two strains of mycorrhizal fungi (UAMH 11541, UAMH 11543) were isolated from roots of an epiphytic orchid in south Florida, Encyclia tampensis; one was acquired from a seedling and one from a mature specimen. Seeds of four epiphytic taxa were subsequently inoculated (separately) with both fungal strains in vitro: E. tampensis, Epidendrum amphistomum, Epidendrum nocturnum, and Prosthechea cochleata. More than one-third of inoculated E. tampensis and E. nocturnum seeds developed leaves in total darkness after 100 days. No significant differences were detected between the two strains on germination, nor any interaction between fungus and seed source (ANOVA, α = 0.05). Using ITS amplification and sequencing, both strains were identified as the teleomorph, Tulasnella irregularis (Basidiomycota: Tulasnellaceae), and both were genetically identical with a high (98%) degree of certainty. Thus, symbiotic germination and ITS sequencing results are in agreement that both strains are indeed the same fungus. This paper is meant to shed additional light into epiphytic orchid-fungal interactions and highlights the need to identify, test (through symbiotic germination) and safeguard mycorrhizal fungi necessary for conservation. 2013 artículo científico 1409-3871 https://www.redalyc.org/articulo.oa?id=44340043013 en http://www.redalyc.org/revista.oa?id=443 Lankesteriana International Journal on Orchidology application/pdf Universidad de Costa Rica Lankesteriana International Journal on Orchidology (Costa Rica) Num.1-2 Vol.13 |
| format | Artículo científico |
| id | redalyc_44340043013 |
| institution | Redalyc |
| language | en |
| publishDate | 2013 |
| publisher | Universidad de Costa Rica |
| spellingShingle | TULASNELLA IRREGULARIS (BASIDIOMYCOTA: TULASNELLACEAE) FROM ROOTS OF ENCYCLIA TAMPENSIS IN SOUTH FLORIDA, AND CONFIRMATION OF ITS MYCORRHIZAL SIGNIFICANCE THROUGH SYMBIOTIC SEED GERMINATION Lawawrence W. Zettler Biología physiology Epidendrum Conservation Basidiomycota ITS sequencing TULASNELLA IRREGULARIS (BASIDIOMYCOTA: TULASNELLACEAE) FROM ROOTS OF ENCYCLIA TAMPENSIS IN SOUTH FLORIDA, AND CONFIRMATION OF ITS MYCORRHIZAL SIGNIFICANCE THROUGH SYMBIOTIC SEED GERMINATION Lawawrence W. Zettler Laura L. Corey Alishia L. Jacks Luke T. Gruender Alyssa M. Lopez Biología physiology Epidendrum Conservation Basidiomycota ITS sequencing Epiphytic orchids remain understudied with respect to their obligate mycorrhizal relationships – a key component of the integrated conservation model. Existing studies have revealed that these plants, like their terrestrial counterparts, commonly associate with ubiquitous basidiomycetes (e.g., Tulasnellaceae); however, few studies have verified their physiological role(s). Two strains of mycorrhizal fungi (UAMH 11541, UAMH 11543) were isolated from roots of an epiphytic orchid in south Florida, Encyclia tampensis; one was acquired from a seedling and one from a mature specimen. Seeds of four epiphytic taxa were subsequently inoculated (separately) with both fungal strains in vitro: E. tampensis, Epidendrum amphistomum, Epidendrum nocturnum, and Prosthechea cochleata. More than one-third of inoculated E. tampensis and E. nocturnum seeds developed leaves in total darkness after 100 days. No significant differences were detected between the two strains on germination, nor any interaction between fungus and seed source (ANOVA, α = 0.05). Using ITS amplification and sequencing, both strains were identified as the teleomorph, Tulasnella irregularis (Basidiomycota: Tulasnellaceae), and both were genetically identical with a high (98%) degree of certainty. Thus, symbiotic germination and ITS sequencing results are in agreement that both strains are indeed the same fungus. This paper is meant to shed additional light into epiphytic orchid-fungal interactions and highlights the need to identify, test (through symbiotic germination) and safeguard mycorrhizal fungi necessary for conservation. 2013 artículo científico 1409-3871 https://www.redalyc.org/articulo.oa?id=44340043013 en http://www.redalyc.org/revista.oa?id=443 Lankesteriana International Journal on Orchidology application/pdf Universidad de Costa Rica Lankesteriana International Journal on Orchidology (Costa Rica) Num.1-2 Vol.13 |
| title | TULASNELLA IRREGULARIS (BASIDIOMYCOTA: TULASNELLACEAE) FROM ROOTS OF ENCYCLIA TAMPENSIS IN SOUTH FLORIDA, AND CONFIRMATION OF ITS MYCORRHIZAL SIGNIFICANCE THROUGH SYMBIOTIC SEED GERMINATION |
| topic | Biología physiology Epidendrum Conservation Basidiomycota ITS sequencing |
| url | https://www.redalyc.org/articulo.oa?id=44340043013 |