Bacterial contamination of automotive fuels in a tropical region: the case of Costa Rica
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| Format: | Artículo científico |
| Langue: | en |
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Universidad de Costa Rica
2009
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| author | Carlos E. Rodríguez-Rodríguez |
| author_facet | Carlos E. Rodríguez-Rodríguez |
| contents | Bacterial contamination of automotive fuels in a tropical region: the case of Costa Rica Carlos E. Rodríguez-Rodríguez Evelyn Rodríguez-Cavallini Rigoberto Blanco Biología Fuel biocorrosion fuel storage tanks microbial contamination sulfate reducing bacteria Microbial contamination of fuel has been the cause of several problems in transportation and storage of these products. Due to the lack of previous studies related to these problems in Costa Rica, bacterial quality was evaluated biannually in automotive fuels stored in the four oil distribution facilities of the Costa Rican Petroleum Refinery (RECOPE). In 12 oil storage tanks, for a total of 96 samples, mesophilic, heterotrophic aerobic/facultative counts (ASTM D6974-04) and identification of bacteria presented in regular gas, premium gas and diesel from the bottom and superior part of the tanks were done; in the samples containing an aqueousphase, sulfate reducing bacteria (SRB) were also quantified by the most probable number technique (MPN), according to the ASTM D4412-84 standard. The higher contamination was shown at the bottom of the tanks (populations up to 104 UFC/l), especially if there was accumulated water, in which case populations reached 108 UFC/l. The most contaminated fuel was diesel (counts up to 104 UFC/l), whereas the less contaminated was premium gas. The less contaminated fuels were from the facilities of La Garita and Barranca, whereas the most contaminated were from Ochomogo. Nevertheless, the quantified populations did not cause significant alteration in quality physicochemical parameters in the samples analyzed. A total of 149 bacterial strains were isolated, 136 (91.3%) Gram positive and 13 (8.7%) Gram negative. The most frequent genera were Staphylococcus (24.0%), Micrococcus (21.9%), Bacillus (18.8%) and Kocuria (11.5%) among Gram positive bacteria and Pseudomonas (7.3%) among Gram negative bacteria. The majority of these genera have been found as fuel contaminants or even as degraders of this kind of products; nevertheless, some species for which their appearance or growth in hydrocarbons have not been described were found with low frequencies. SRB were present in counts up to 105 MPN/l in 42.9% of water containing samples (including all from diesel tanks), indicating biocorrosion processes risk in fuel transport and storage systems. From the findings in this study it is recommended to give a frequent maintenance to fuel containers, based on continuous drainage and removal of accumulated water, antimicrobial agent addition and microbial quality monitoring in country's fuels. 2009 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44911876004 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.3 Vol.57 |
| format | Artículo científico |
| id | redalyc_44911876004 |
| institution | Redalyc |
| language | en |
| publishDate | 2009 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Bacterial contamination of automotive fuels in a tropical region: the case of Costa Rica Carlos E. Rodríguez-Rodríguez Biología Fuel biocorrosion fuel storage tanks microbial contamination sulfate reducing bacteria Bacterial contamination of automotive fuels in a tropical region: the case of Costa Rica Carlos E. Rodríguez-Rodríguez Evelyn Rodríguez-Cavallini Rigoberto Blanco Biología Fuel biocorrosion fuel storage tanks microbial contamination sulfate reducing bacteria Microbial contamination of fuel has been the cause of several problems in transportation and storage of these products. Due to the lack of previous studies related to these problems in Costa Rica, bacterial quality was evaluated biannually in automotive fuels stored in the four oil distribution facilities of the Costa Rican Petroleum Refinery (RECOPE). In 12 oil storage tanks, for a total of 96 samples, mesophilic, heterotrophic aerobic/facultative counts (ASTM D6974-04) and identification of bacteria presented in regular gas, premium gas and diesel from the bottom and superior part of the tanks were done; in the samples containing an aqueousphase, sulfate reducing bacteria (SRB) were also quantified by the most probable number technique (MPN), according to the ASTM D4412-84 standard. The higher contamination was shown at the bottom of the tanks (populations up to 104 UFC/l), especially if there was accumulated water, in which case populations reached 108 UFC/l. The most contaminated fuel was diesel (counts up to 104 UFC/l), whereas the less contaminated was premium gas. The less contaminated fuels were from the facilities of La Garita and Barranca, whereas the most contaminated were from Ochomogo. Nevertheless, the quantified populations did not cause significant alteration in quality physicochemical parameters in the samples analyzed. A total of 149 bacterial strains were isolated, 136 (91.3%) Gram positive and 13 (8.7%) Gram negative. The most frequent genera were Staphylococcus (24.0%), Micrococcus (21.9%), Bacillus (18.8%) and Kocuria (11.5%) among Gram positive bacteria and Pseudomonas (7.3%) among Gram negative bacteria. The majority of these genera have been found as fuel contaminants or even as degraders of this kind of products; nevertheless, some species for which their appearance or growth in hydrocarbons have not been described were found with low frequencies. SRB were present in counts up to 105 MPN/l in 42.9% of water containing samples (including all from diesel tanks), indicating biocorrosion processes risk in fuel transport and storage systems. From the findings in this study it is recommended to give a frequent maintenance to fuel containers, based on continuous drainage and removal of accumulated water, antimicrobial agent addition and microbial quality monitoring in country's fuels. 2009 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44911876004 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.3 Vol.57 |
| title | Bacterial contamination of automotive fuels in a tropical region: the case of Costa Rica |
| topic | Biología Fuel biocorrosion fuel storage tanks microbial contamination sulfate reducing bacteria |
| url | https://www.redalyc.org/articulo.oa?id=44911876004 |