Lactonas sesquiterpénicas de las plantas Viguiera sylvatica y Decachaeta thieleana (Asteraceae) modulan la producción de óxido nítrico y la fagocitosis de macrófagos RAW
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| Natura: | Artículo científico |
| Lingua: | es |
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Universidad de Costa Rica
2008
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| _version_ | 1876452610522742784 |
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| author | Omar A. Dupuy L. |
| author_facet | Omar A. Dupuy L. |
| contents | Lactonas sesquiterpénicas de las plantas Viguiera sylvatica y Decachaeta thieleana (Asteraceae) modulan la producción de óxido nítrico y la fagocitosis de macrófagos RAW Omar A. Dupuy L. José A. Bonilla V. Renato Murillo Biología thieleanin Asteraceae millerenolide immunomodulation sesquiterpene lactones Sesquiterpene lactones of the plants Viguiera sylvatica and Decachaeta thieleana (Asteraceae) modulate nitric oxide production and phagocytosis of macrophages cell line RAW. Different species of the Asteraceae family are a potential source of sesquiterpene lactones with anti-inflammatory properties. Macrophages play a central role in the regulation of immune responses. In the present study, the in vitro effect of two sesquiterpene lactones, a millerenolide and a thieleanin, was assessed by measuring the production of nitric oxide (NO) by cell line RAW (murine macrophages) using the Griess reagent. Additionally, the effect of these sesquiterpene lactones on phagocytic capacity of latex particles and the reduction of nitroblue tetrazolium (NBT) were evaluated microscopically. Treatment of macrophages with >2.5 µg/ml of both sesquiterpene lactones, reduced the production of NO. A decreased number of macrophages able to reduce NBT were observed when these cells were treated with 3 µg/ml of millerenolide or 7.5 µg/ml of thieleanin. Treatment of macrophages with 4 µg/ml of millerenolide or 7.5 µg/ml of thieleanin, reduced the phagocytic capacity of macrophages. Cytotoxic effects on the macrophages were only observed when the concentration was increased to 8 µg/ml of millerenolide or 25 µg/ml of thieleanin. Our results suggest that these sesquiterpene lactones could be useful compounds in the elaboration of anti-inflammatory drugs. Rev. Biol. Trop. 56 (3): 1063-1073. Epub 2008 September 30. 2008 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44918834008 es http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.3 Vol.56 |
| format | Artículo científico |
| id | redalyc_44918834008 |
| institution | Redalyc |
| language | es |
| publishDate | 2008 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Lactonas sesquiterpénicas de las plantas Viguiera sylvatica y Decachaeta thieleana (Asteraceae) modulan la producción de óxido nítrico y la fagocitosis de macrófagos RAW Omar A. Dupuy L. Biología thieleanin Asteraceae millerenolide immunomodulation sesquiterpene lactones Lactonas sesquiterpénicas de las plantas Viguiera sylvatica y Decachaeta thieleana (Asteraceae) modulan la producción de óxido nítrico y la fagocitosis de macrófagos RAW Omar A. Dupuy L. José A. Bonilla V. Renato Murillo Biología thieleanin Asteraceae millerenolide immunomodulation sesquiterpene lactones Sesquiterpene lactones of the plants Viguiera sylvatica and Decachaeta thieleana (Asteraceae) modulate nitric oxide production and phagocytosis of macrophages cell line RAW. Different species of the Asteraceae family are a potential source of sesquiterpene lactones with anti-inflammatory properties. Macrophages play a central role in the regulation of immune responses. In the present study, the in vitro effect of two sesquiterpene lactones, a millerenolide and a thieleanin, was assessed by measuring the production of nitric oxide (NO) by cell line RAW (murine macrophages) using the Griess reagent. Additionally, the effect of these sesquiterpene lactones on phagocytic capacity of latex particles and the reduction of nitroblue tetrazolium (NBT) were evaluated microscopically. Treatment of macrophages with >2.5 µg/ml of both sesquiterpene lactones, reduced the production of NO. A decreased number of macrophages able to reduce NBT were observed when these cells were treated with 3 µg/ml of millerenolide or 7.5 µg/ml of thieleanin. Treatment of macrophages with 4 µg/ml of millerenolide or 7.5 µg/ml of thieleanin, reduced the phagocytic capacity of macrophages. Cytotoxic effects on the macrophages were only observed when the concentration was increased to 8 µg/ml of millerenolide or 25 µg/ml of thieleanin. Our results suggest that these sesquiterpene lactones could be useful compounds in the elaboration of anti-inflammatory drugs. Rev. Biol. Trop. 56 (3): 1063-1073. Epub 2008 September 30. 2008 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44918834008 es http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.3 Vol.56 |
| title | Lactonas sesquiterpénicas de las plantas Viguiera sylvatica y Decachaeta thieleana (Asteraceae) modulan la producción de óxido nítrico y la fagocitosis de macrófagos RAW |
| topic | Biología thieleanin Asteraceae millerenolide immunomodulation sesquiterpene lactones |
| url | https://www.redalyc.org/articulo.oa?id=44918834008 |