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| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad de Costa Rica
2009
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| Online Access: | https://www.redalyc.org/articulo.oa?id=44918950013 |
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| _version_ | 1866585188938547200 |
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| author | Rafael Vega |
| author_facet | Rafael Vega |
| contents | Histology of somatic embryogenesis in rice (Oryza sativa cv. 5272) Rafael Vega Nelly Vásquez Ana M. Espinoza Andrés M. Gatica Marta Valdez-Melara Biología rice histology Costa Rica Oryza sativa morphogenesis Rice (Oryza sativa cv. 5272) embryogenic calli were obtained from mature zygotic embryos culture on Murashige & Skoog (1962) medium supplemented with 2.5 mg/l 2,4- dichlorophenoxyacetic acid. Histological analysis of somatic embryogenesis revealed that after two weeks of culture of explants on the callus induction medium, somatic embryo development began with a cluster of proembryogenic cells in the peripheral region of the calli. The outer cell layer of embryogenic calli consisted of small and isodiametric cells with a dense cytoplasm and a prominent nucleus and nucleolus; whereas the inner cell layer is composed of large cells with small nucleus and large vacuole. These embryogenic cells underwent a series of organized divisions and formed the proembryo with a well-defined protodermis. 2009 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44918950013 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.57 |
| format | Artículo científico |
| id | redalyc_44918950013 |
| language | en |
| publishDate | 2009 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Histology of somatic embryogenesis in rice (Oryza sativa cv. 5272) Rafael Vega Biología rice histology Costa Rica Oryza sativa morphogenesis Histology of somatic embryogenesis in rice (Oryza sativa cv. 5272) Rafael Vega Nelly Vásquez Ana M. Espinoza Andrés M. Gatica Marta Valdez-Melara Biología rice histology Costa Rica Oryza sativa morphogenesis Rice (Oryza sativa cv. 5272) embryogenic calli were obtained from mature zygotic embryos culture on Murashige & Skoog (1962) medium supplemented with 2.5 mg/l 2,4- dichlorophenoxyacetic acid. Histological analysis of somatic embryogenesis revealed that after two weeks of culture of explants on the callus induction medium, somatic embryo development began with a cluster of proembryogenic cells in the peripheral region of the calli. The outer cell layer of embryogenic calli consisted of small and isodiametric cells with a dense cytoplasm and a prominent nucleus and nucleolus; whereas the inner cell layer is composed of large cells with small nucleus and large vacuole. These embryogenic cells underwent a series of organized divisions and formed the proembryo with a well-defined protodermis. 2009 artículo científico 0034-7744 https://www.redalyc.org/articulo.oa?id=44918950013 en http://www.redalyc.org/revista.oa?id=449 Revista de Biología Tropical application/pdf Universidad de Costa Rica Revista de Biología Tropical (Costa Rica) Num.1 Vol.57 |
| title | Histology of somatic embryogenesis in rice (Oryza sativa cv. 5272) |
| topic | Biología rice histology Costa Rica Oryza sativa morphogenesis |
| url | https://www.redalyc.org/articulo.oa?id=44918950013 |